摘要
目的观察中药鼻敏温阳方对肾阳虚证变应性鼻炎大鼠鼻黏膜嗜酸性粒细胞的影响。方法 70只雄性健康大鼠,制备肾阳虚型变应性鼻炎模型,然后分为空白、模型、西替利嗪、大剂量中药、小剂量中药5组。除空白组为10只外,其余每组均为15只动物。干预试验结束后,处死动物,游离鼻中隔黏膜,常规切片,HE染色,观察比较各组的组织学改变及嗜酸性粒细胞浸润情况。结果模型组鼻黏膜嗜酸性粒细胞计数明显高于西替利嗪组、大剂量中药组、小剂量中药组和空白组,而大剂量中药组者又明显低于西替利嗪组和小剂量中药组,西替利嗪组与小剂量中药组的浸润细胞数差异不具有统计学意义。结论鼻敏温阳方治疗肾阳亏虚型变应性鼻炎,可能通过减轻鼻黏膜嗜酸性粒细胞的聚集及活化而阻断变态反应的进程。
Objective To investigate the effect of Chinese herbal compound Bimin Wenyang formula （BWF） on the eosinophils infiltrating in the nasal mucous membranes of rats with experiment allergic rhinitis at the pattern of Kidney Yang deficiency. Methods In this study, 60 healthy male rats were chosen to prepare the model of allergic rhinitis at the pattern of Kidney Yang deficiency, with another 10 rates taken as healthy controls （HC）. Then, these modeled rats were divided into four groups, i.e. modeling group （MG）, cetirizine treating group （CTG）, larger dose of BWF treating group （TGL） and smaller dose of BWF treating group （TGS）, with 15 animals in each group of these modeling rats, and 5 groups in total when HC being taken together. All these animals were treated by corresponding procedures respectively as designed before the experiment procedures undergoing, except those of HC. When the intervening course finished, all these rats were put into death to collect mucosa^1 samples from their nasal septum respectively to prepare tissue sections for histological observation of eosinophils infiltrating in nasal mucosa, carried out under optical microscope in a comparative way among various groups. Results It was shown from this study that the number of the eosinophils infiltrating in nasal mucosa of rats in modeling group was the highest, significantly higher than that in CG, TGL, TGS and HC （P〈0.01）, while this number of TGL was markedly lower than that of CG and TGS （P〈0.01）. However, there was no significant difference between CG and TGS in the infiltrating number of eosinophils in nasal mucosa （P〉0.05）. Conclusions The effect of BWF on allergic rhinitis at the pattern of Kidney Yang deficiency may be brought about via alleviating aggregation and activation of the eosinophils treating in nasal mucosa to prevent allergic reaction from attacking.
出处
《中国中西医结合耳鼻咽喉科杂志》
2011年第2期65-68,共4页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
基金
科技部"十一五"国家科技支撑计划(2007BAI2008037)
江苏省中医药管理局科研基金资助(H05049)
