摘要
目的:探讨骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)移植对舍格伦综合征(Sjǒgren’s syndrome)大鼠模型的治疗作用及其在下颌下腺(submandibular gland,SMG)内存活、迁徙及分化情况。方法:建立大鼠舍格伦综合征动物模型;分离、培养大鼠BMSCs并对其进行鉴定和标记;下颌下腺局部注射移植经绿色荧光蛋白标记的BMSCs;测定大鼠静态唾液总流率,记录正常组、模型组、模型治疗组及模型治疗对照组大鼠每日饮水量;荧光显微镜下观察移植干细胞在模型治疗组和模型治疗对照组下颌下腺内的存活、迁徙及分化情况。所得实验数据采用SPSS12.0软件包进行t检验。结果:模型治疗组静态唾液总流率和每天饮水量与模型治疗对照组相比,差异有统计学意义(P<0.05)。经标记的骨髓间充质干细胞移植后第1周,BMSCs主要分布于针道附近,第2周主要分布于腺泡之间的间质中,第4周开始在下颌下腺其他区域出现。术后第1周,免疫组化染色移植干细胞未见淀粉酶表达,第8周可见在少量移植细胞的胞质内有淀粉酶表达,具备了类似腺泡细胞的分泌功能。模型治疗对照组未发现以上现象。结论:BMSCs移植对舍格伦综合征大鼠有一定治疗作用。
PURPOSE： To study the curative effects,survival rate,migration and differentiation of bone mesenchymal stem cells（BMSCs） transplanted into rats with Sjǒgren＇s syndrome.METHODS： Model of Sjǒgren＇s syndrome was created in rats.BMSCs were isolated and cultured by using the preceding method.Then the pretreated BMSCs were identified and labeled by enhanced green fluorescent protein.BMSCs marked with enhanced green fluorescent protein（EGFP） or PBS were injected into the SMG of the Sjǒgren＇s syndrome rats via open surgery.Total static saliva flow rate was determined in rats.The daily amount of water in the normal group,the model group,the model treatment group and the model treatment control group was recorded.The survival rate,migration and differentiation of the BMSCs transplanted in the treatment group under fluorescence microscope was recorded.Student＇s t test was used for data analysis using SPSS 12.0 software package.RESULTS： Compared with the model treatment control group,the total static saliva flow rate of the model treatment group increased significantly,and the water they drank decreased significantly（P〈0.05）.In addition,BMSCs were distributed along the injection tract mostly in the first week,then BMSCs were mainly distributed in the stroma between the acinar in the second week and were distributed over other areas four weeks later.Immunohistochemical staining of amylzyme was not observed at the first week after transplantation.And at the 8th week the expression of amylzyme in the cytoplasm of the transplanted BMSCs was observed by immunohistochemical only in the model treatment group.CONCLUSION： Transplantation of BMSCs has certain treatment effect on Sjǒgren＇s syndrome.
出处
《上海口腔医学》
CAS
CSCD
2011年第2期152-158,共7页
Shanghai Journal of Stomatology
基金
温州市科技局重大项目科研基金(Y20080090)~~
关键词
骨髓间充质干细胞
舍格伦综合征
细胞移植
Bone mesenchymal stem cells
BMSC
Sjǒgren＇s syndrome
Cell transplantation
