摘要
目的探讨应用细胞DNA定量分析方法鉴别诊断良、恶性乳腺包块的价值，为临床制定手术方法和预后方案提供依据。方法488例乳腺包块住院患者被纳入研究对象，诊断医师进行针吸穿刺，然后将样品制成2张薄层涂片。1张涂片做巴氏染色，用于常规细胞学检查，另一张经Feulgen染色，用自动细胞DNA定量分析系统检测。每例均进行术后病理观察。结果488例乳腺肿物经病理证实，164例为乳腺良陛肿瘤，324例为恶性肿瘤。常规细胞学方法对乳腺肿瘤的灵敏度为91．4％（296／324）、特异性为92．7％（152／164）；而细胞DNA定量分析方法的灵敏度为90．1％（292／324）、特异性为100．0％（164／164）。常规细胞学方法阳性预测值为96．1％（296／308），阴性预测值为84．4％（152／180），而细胞DNA定量分析方法中阳性预测值为100．0％（292／292），阴性预测值为83．7％（164／196）。结论细胞DNA定量分析可用于乳腺肿块术前诊断中恶性程度的判断。
Objective To explore the application of quantitative DNA analysis in differential diagnosis of benign and malignant breast masses to aid the surgery plan formation. Methods Four hundred and eighty-eight patients with breast mass were enrolled into this study. Tissues of breast mass in patients were gained by fine-needle aspiration puncture. Two sections were macle from each sample, one was stained by Papanicolaou for regular Cytology analysis and another was stained with Feulgen for quantitative DNA analysis. Pathological results were confirmed in each case after surgery. Results One hundred and sixty-four cases were classified as patients with benign neoplasm, while the other 324 eases were classified as malignant neoplasm, according to the pathological examination results. The sensibility and specificity were 91.4% （296/324） and 92. 7% （ 152/164 ） for regular cytological method, 90. 1% （ 292/324 ） and 100. 0% （ 164/164 ） for quantitative DNA analysis method. Meanwhile the positive predictive and negtive value of quantitative DNA analysis was 100. 0% （ 292/292 ） and 83.7% （ 164/164 ）, of which regular cytological methods were 96. 1% （ 296/308 ） and 84.4% （152/180）. Conclusion The quantitative DNA analysis might assistant differential diagnosis of benign and malignant breast tumor.
出处
《中国综合临床》
2011年第5期449-451,共3页
Clinical Medicine of China
基金
河北省科学技术研究与发展计划项目（09276174）
关键词
乳腺肿瘤
DNA定量分析
异倍体细胞
Breast masses
Quantitative DNA analysis
Heteroploid cells
