摘要
目的:通过构建牙本质-牙髓复合体损伤修复动物模型,研究基质细胞衍生因子1α及其受体CXCR4(SDF-1α/CXCR4)在其损伤修复过程中的表达。方法:制备大鼠牙本质-牙髓损伤模型,通过免疫组织化学染色、PCR技术分别对损伤后0、6h和1、3、7d及正常大鼠牙髓标本中SDF-1α/CXCR4进行检测。结果:正常大鼠组牙髓中没有发现SDF-1α、CXCR4阳性表达,实验大鼠组损伤6h后少量的成牙本质细胞和前期牙本质开始出现较弱阳性表达,损伤1d后成牙本质细胞阳性表达增强,损伤3d后牙髓成纤维细胞出现阳性表达,损伤7d后未分化间充质细胞出现阳性表达,大鼠牙髓损伤后SDF-1α、CXCR4随着时间的增加阳性表达程度明显增强。图像分析结果显示,SD大鼠牙本质-牙髓复合体损伤后牙髓中SDF-1α、CXCR4的表达(灰度值)除Oh外其余各组均高于对照组(P<0.05)。PCR结果显示,损伤后大鼠牙髓中SDF-1α、CXCR4均符合产物设计大小。其表达(灰度值)除Oh外其余各组均高于对照组(P<0.05)。结论:大鼠牙本质-牙髓复合体损伤后,前期牙本质、成牙本质细胞、牙髓成纤维细胞和未分化间充质细胞对SDF-1α、CXCR4的表达呈时间依赖性,提示SDF-1α、CXCR4可能参与了修复性牙本质的形成。
AIM：To investigate the expression and effect of stomal cell derived factor 1 alpha（SDF-1α） and its receptor CXCR4 in the rehabilitation process of dentin-dental pulp injury by establishing an animal model of dentin-dental pulp injury rehabilitation.METHODS：A rat model of dental pulp-dentin injury was established.SDF-1αand CXCR4 expression in dental pulp samples at the moment of 0h,6h,1 d,3d,7d after injury and in normal pulp was detected by immunohistochemical staining（IHC） and RT-PCR.RESULTS：No positive staining of SDF-1αand CXCR4 was detected in normal dental pulp samples by IHC.6 h after injury,weak expression of SDF-1αand CXCR4 could be detected in a small number of dentin cells and in predentin.Meanwhile,positive staining was found in fibroblasts and undifferentiated mesenchymal cells during 1 d to 7d,staining became significantly enhanced with the time course.RT-PCR revealed that SDF -1αand CXCR4 mRNA expression significantly increased time-dependently after pulp injury（P0.05）.CONCLUSION： After injury,SDF-1αand CXCR4 expression in rat dental pulp-dentin complex,including predentin,dentin cells, fibroblasts and undifferentiated mesenchymal cells,significantly increased during early phase of repair.The results indicated that SDF-1αand CXCR4 might participate in the formation of reparative dentin.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2011年第4期187-191,共5页
Chinese Journal of Conservative Dentistry
基金
陕西省科学技术发展计划项目(S2009SF498)
