摘要
从植物乳杆菌B-28的代谢产物中分离纯化植物乳杆菌素,以蜡样芽胞杆菌为指示菌,通过测定指示菌细胞结构和细胞中APT、离子量的变化,分析探讨植物乳杆菌素的抑菌机理。研究结果表明:当植物乳杆菌素作用于蜡样芽胞杆菌时,胞内ATP呈现迅速下降趋势;并迅速释放出K+和Na+,而且植物乳杆菌素的质量分数与K+和Na+释放量基本呈现线性正相关。随着植物乳杆菌素添加量的增加,蜡样芽胞杆菌细胞裂解率在逐渐提高。而且电镜观察发现:植物乳杆菌素作用的蜡样芽胞杆菌的细胞壁边缘模糊,菌体细胞变形,部分细胞壁缺失,可看到胞内物质的损失。
The plantarcin was screened by Lactobacillus plantarum B-28.The Bacillus cereus was the indicator bacteria.The antibacterial mechanism of the plantarcin was investigated through the changes of ATP level,ionic content and structure of cell.The results showed： As plantarcin which was screened by Lactobacillus plantarum B-28 was added,intracellular ATP levels in energized cells of the Bacillus cereus dropped quickly and then tended to be stable,however,extracellular ATP level increased during incubation in the present of plantaricin.The indictor＇s cell lysis rate increased when the content of plantaricin was increased.After plantarcin was added,intracellar K＋ and Na＋ released quickly.It seemed there was a linear correlation between total amount of ions released and the concentration of plantaricin.Bacillus cereus were treated by plantaricin,it was clearly seen that its cells wall appeared irregular at the edges by electron microscopy.At that time,their cells were cytomorphosis,cell walls were partially damaged,and intracellular materials were lost.
出处
《中国乳品工业》
CAS
北大核心
2011年第5期31-33,36,共4页
China Dairy Industry
关键词
植物乳杆菌素
抑菌
机理
plantaricin
antibacterial
mechanism.
