期刊文献+

体外诱导BMSCs向软骨分化的方法研究进展 被引量:5

PROGRESS OF METHODS OF INDUCING BONE MARROW MESENCHYMAL STEM CELLS INTO CHONDROCYTES IN VITRO
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摘要 目的全面了解目前体外诱导BMSCs向软骨分化的方法,为软骨组织工程研究提供参考。方法广泛查阅近年来有关软骨组织工程中诱导BMSCs向软骨分化的文献,并进行综合分析。结果目前BMSCs诱导成软骨方法主要是添加外源性生长因子,其中TGF-β家族被公认为最重要的诱导和调节因子。其他重要的诱导方法包括添加多种化学因子、物理因素、转基因技术和微环境诱导等方法,但这些方法仍存在诱导效率低、诱导效果不稳定的问题。结论诱导方法的进展促进了BMSCs在软骨组织工程中的应用,建立更高效、简便、安全的诱导方法仍是软骨组织工程领域重要研究课题之一。 Objective To review the research progress of the current methods of inducing bone marrow mesenchymal stem cells(BMSCs) to chondrogenic differentiation in vitro so as to provide references for researches in cartilage tissue engineering.Methods Various methods of inducing BMSCs differentiation into the chondrogenic lineage in vitro in recent years were extensively reviewed and analyzed.Results Adding exogenous growth factors is still the mainly method of inducing BMSCs differentiation into the chondrogenic lineage;among the members,transforming growth factor β(TGF-β) family is recognized as the most important chondrogenic induction factor.Other important inducing factors include various chemical factors,physical factors,transgenic methods,and the microenvironmental induction.But the problems of low inducing efficiency and unstable inducing effects still exist.Conclusion The progress of chondrogenic induction of BMSCs promotes its utilization in cartilage tissue engineering.Further researches are needed for establishing more efficient,simpler,and safer inducing methods
出处 《中国修复重建外科杂志》 CAS CSCD 北大核心 2011年第5期618-623,共6页 Chinese Journal of Reparative and Reconstructive Surgery
基金 国家自然科学基金青年科学基金资助项目(30801192) 北京市自然科学基金资助项目(7102134) 高等学校博士学科点专项科研基金(200800231078)~~
关键词 软骨组织工程 BMSCS 成软骨分化 生长因子 诱导 Cartilage tissue engineering Bone marrow mesenchymal stem cells Chondrogenic differentiation Growth factor Induction
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参考文献79

  • 1Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol, 2004, 36(4): 568-584.
  • 2Canalis E, Economides AN, Gazzerro E. Bone morphogenetic proteins, their vantagonists, and the skeleton. Endocr Rev, 2003, 24(2): 218-235.
  • 3Fan H, Hu Y, Qin L, et al. Porous gelatin-chondroitin-hyaluronate tricopolymer scaffold containing microspheres loaded with TGF-betal induces differentiation of mesenchymal stem cells in vivo for enhancing cartilage repair. J Biomed Mater Res, 2006, 77(4): 785-794.
  • 4Xia W, Jin YQ, Kretlow JD, et al. Adenoviral transduction of hTGF- betal enhances the chondrogenesis of bone marrow derived stromal cells. Biotechnol Lett, 2009, 31(5): 639-646.
  • 5Ferguson CM, Schwarz EM, Reynolds PR, et al. Smad2 and 3 mediate transforming growth factor-betal-induced inhibition of chondrocyte maturation. Endocrinology, 2000, 141(12): 4728-4735.
  • 6Johnstone B, Hering TM, Caplan AI, et al. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res, 1998, 238(1): 265-272.
  • 7Mackay AM, Beck SC, Murphy JM, et al. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng, 1998, 4(4): 415-428.
  • 8Barry F, Boynton RE, Liu B, et al. Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components. Exp Cell Res, 2001, 268(2): 189-200.
  • 9Mueller MB, Fischer M, Zellner J, et al. Hypertrophy in mesenchymal stem cell chondrogenesis: effect of TGF-beta isoforms and chondrogenic conditioning. Cells Tissues Organs, 2010, 192(3): 158-166.
  • 10Weiss S, Hennig T, Bock R, et al. Impact of growth factors and PTHrP on early and late chondrogenic differentiation of human mesenchymal stem cells. J Cell Physiol, 2010, 223 ( 1): 84-93.

同被引文献130

  • 1Saurabh Pratap Singh,Naresh Kumar Tripathy,Soniya Nityanand.Comparison of phenotypic markers and neural differentiation potential of multipotent adult progenitor cells and mesenchymal stem cells[J].World Journal of Stem Cells,2013,5(2):53-60. 被引量:10
  • 2周晓中,张文智,吕维佳,董启榕,陈振腾,梁智仁.多聚乙醇酸-羟基磷灰石复合体为支架的骨髓基质细胞修复兔关节软骨缺损[J].中华创伤杂志,2005,21(8):617-620. 被引量:9
  • 3Outani H,Okada M,Hiramatsu K. Induction of chondrogenic cel s from dermal fibroblast culture by defined factors does not involve a pluripotent state[J].Biochemical and Biophysical Research Communications,2011,(03):607-612.
  • 4Huang AH,Yeger-McKeever M,Stein A,Mauck RL. Tensile properties of engineered cartilage formed from chondrocyte-and MSC-laden hydrogels[J].Osteoarthritis and Cartilage,2008,(09):1074-1082.
  • 5Eleswarapu SV,Leipzig ND,Athanasiou KA. Gene expression of single articular chondrocytes[J].Cel Tissue Res,2007,(01):43-54.
  • 6Koay EJ,Athanasiou KA. Development of serum-free,chemical y defined conditions for human embryonic stem cel -derived fibrochondrogenesis[J].Tissue Eng Part A,2009,(08):2249-2257.
  • 7Solorio LD,Fu AS,Hernandez-Irizarry R. Chondrogenic differentiation of human mesenchymal stem cel aggregates via control ed release of TGF-beta1 from incorporated polymer microspheres[J].BioMedical Materials Research Part A,2010,(03):1139-1144.
  • 8Stephens P,Genever P. Non-epithelial oral mucosal progenitor cel populations[J].Oral Diseases,2007.1-10.
  • 9Tiede S,Kloepper JE,Bodò E. Hair fol icle stem cel s:Walking the maze[J].Eur J Cel Biol,2007,(07):355-376.
  • 10Huelsken J,Vogel R,Erdmann B. Beta-catenin controls hair fol icle morphogenesis and stem cel differentiation in the skin[J].Cell,2001.533-545.

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