摘要
Background OGR1 was found as a G-protein coupled receptor （GPCR） and proton sensor. Our previous studies have found that OGR1 has inhibitory effect on the metastasis of prostate cancer. In order to investigate the roles of OGR1 gene in the biological activities of ovarian cancer, we studied the OGR1 effects on ovarian cancer cells, HEY cells.Methods OGR1 gene was transfected into HEY cell, in which endogenous expression is low. OGR1-overxepressed cells and vector-transfected cells were compared in different assays. Western blotting was employed to confirm the high expression level of OGR1. Cell proliferation was determined by MTT assay and cell doubling time assay. Cell migration assay （transwell assay） and cell adhesion assay were performed to determine the migration and adhesion potential of cells. Student＇s t test was employed for statistical analysis.Results Proliferation of OGR1-overexpressed cells was significantly reduced （P ＜0.01）; cell migration was significantly inhibited in the OGR1-transfected cells （P ＜0.01）; cell adhesion to extracellular matrix including fibronectin, vitronectin,collagen Ⅰ/Ⅳ was significantly increased （P ＜0.01）.Conclusions OGR1 expression in human ovarian cancer cells significantly inhibited the cell proliferation and migration,but significantly enhanced cell adhesion to the extracellular matrix. It indicated that OGR1 may be a tumor suppressor gene for ovarian cancer.
Background OGR1 was found as a G-protein coupled receptor （GPCR） and proton sensor. Our previous studies have found that OGR1 has inhibitory effect on the metastasis of prostate cancer. In order to investigate the roles of OGR1 gene in the biological activities of ovarian cancer, we studied the OGR1 effects on ovarian cancer cells, HEY cells.Methods OGR1 gene was transfected into HEY cell, in which endogenous expression is low. OGR1-overxepressed cells and vector-transfected cells were compared in different assays. Western blotting was employed to confirm the high expression level of OGR1. Cell proliferation was determined by MTT assay and cell doubling time assay. Cell migration assay （transwell assay） and cell adhesion assay were performed to determine the migration and adhesion potential of cells. Student＇s t test was employed for statistical analysis.Results Proliferation of OGR1-overexpressed cells was significantly reduced （P ＜0.01）; cell migration was significantly inhibited in the OGR1-transfected cells （P ＜0.01）; cell adhesion to extracellular matrix including fibronectin, vitronectin,collagen Ⅰ/Ⅳ was significantly increased （P ＜0.01）.Conclusions OGR1 expression in human ovarian cancer cells significantly inhibited the cell proliferation and migration,but significantly enhanced cell adhesion to the extracellular matrix. It indicated that OGR1 may be a tumor suppressor gene for ovarian cancer.
基金
This research was supported by the grants from the National Natural Science Foundation of China （No. 30973175）, Scientific Research Foundation for Returning Scholars of Education Ministry of China （No. 0601-18920006）, Scientific and Technological Research Foundation of Shaanxi Province （No. 2007K09-09）, and the Clinical Research Fundation of First Hospital of Xi＇an Jiaotong University of China.
