摘要
目的对幽门螺杆菌（Ｈｐ）的两种形态进行研究。方法采用细菌培养、电镜观察及ＰＣＲ扩增的ＤＮＡ多形性印迹分析等技术，观察幽门螺杆菌二种形态的转变。结果随着培养时间的延长，Ｈｐ逐渐由螺旋状体转变为圆球体。发生Ｌ型变异圆球体可分为二类，一类具有鞭毛且细胞膜完整，这一类圆球体可能具有活性；另一类圆球体系退行性变。Ｈｐ圆球体与螺旋状体的ＤＮＡ谱十分相似，并且两者都分泌过氧化氢酶、碱性磷酸酶及酸性磷酸酶。结论Ｈｐ圆球体仍具有螺旋状体的诸多生物学共性，至少部分圆球体可能具有活性。因而，在Ｈｐ的传播及相关疾病的治疗复发过程中起重要作用。
Objective The dimorphism (spiral and coccoid forms) of
Helicobacter pylori (Hp) were investigated. Methods Culture, electronmicroscopy and PCRbased
random amplified polymorphism DNA(RAPD) fingerprinting were used. Results Morphological
conversion from spiral to coccoid forms occurred with prolongation of culture. Part of coccoid
forms had intact cellular membranes and flagella indicating they were likely to be viable.
However, some coccoid forms were degenerative. Catalase, alkaline phosphatase and acid
phosphatase were positive for both spiral and coccoid forms. RAPD showed similar DNA
patterns. Conclusion This study shows that at least part of Hp coccoid forms may be viable and
thus may play an important role in the transmission of and be partly responsible for
recrudescence of Hp infection after antimicrobial treatments.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1999年第4期230-232,共3页
Chinese Journal of Digestion
