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江苏省胃癌高发区幽门螺杆菌感染与胃癌、基因突变之间的关系 被引量:16

The relationship between Helicobacter pylori infection, gene mutation and gastric carcinoma in gastric cancer prevalent areas of Jiangsu Province
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摘要 目的研究江苏省胃癌高发区幽门螺杆菌(Hp)感染及其毒力因子与胃癌、基因突变之间的关系。方法用酶链免疫吸附测定(ELISA)、免疫印迹及聚合酶链反应单链构象多态性分析(PCRSSCP)等方法,分析了50例胃癌及50例配对胃炎患者的Hp感染、Hp的细胞毒素相关蛋白(CagA)、空泡细胞毒素(VacA)情况及胃粘膜p53基因第5~8外显子的突变。结果胃癌组中Hp阳性率及CagA阳性率均显著高于胃炎组,p53基因突变与Hp感染有非常显著的相关性,而与CagA、VacA的表达无关。结论Hp(尤其是CagA阳性菌株)感染能增加胃癌的发病风险,在Hp致癌过程中p53等基因突变可能起了关键的作用,研究结果为根治Hp以预防胃癌发生提供了实验依据。 Objective To investigate the relationship botween Hp(Helicobacter pylori) infection and gene mutation, in gastric carcinoma prevalent areas of Jiangsu Province. Methods We studied the prevalence of Hp infection, the status of CagA and VacA of Hp, and p53 exon 5-8 mutations in tissues of 50 gastric carcinoma patients and 50 gastritis patients by the methods of PCRSSCP, immunoblotting and ELISA. Results The positive rates of Hp infection and CagA expression in gastric carcinoma group were significantly higher than those in control group. p53 mutation was significantly associated with Hp infection, but not with the CagA and VacA expression. Conclusions Hp infection, especially the CagA positive strain, increases the risk of gastric carcinoma, in which p53 mutation may play an important role. The above results suggest that eradication of Hp might prevent the occurrence of gastric carcinoma.
出处 《中华消化杂志》 CAS CSCD 北大核心 1999年第4期236-238,共3页 Chinese Journal of Digestion
关键词 胃癌 幽门螺杆菌 CAGA 空泡细胞病毒 P53突变 Gastric carcinomaHpCagAVacAp53 mutation
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参考文献3

  • 1梁后杰 刘维文 等.幽门螺杆菌培养滤液诱导胃粘膜细胞DNA损伤[J].中华消化杂志,1995,15:21-22.
  • 2Chang K H,Med J,1997年,38卷,117页
  • 3梁后杰,中华消化杂志,1995年,15卷,增,21页

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