摘要
利用ISSR和RAPD分子标记技术对23份伯乐树材料进行遗传多样性研究。结果表明,筛选出的13条ISSR和10条RAPD引物分别得到79条和57条扩增带,其中多态性条带分别为50条和33条,多态性条带比率分别为63.29%和57.89%;ISSR和RAPD检测的有效等位基因数分别为1.4036和1.3601,基因多样性为0.2305和0.2115,Shannon信息指数为0.3405和0.3145;分析表明23份伯乐树之间具有比较丰富的遗传变异。对比ISSR和RAPD在PCR反应中的稳定性和检测变异的能力表明,对于试验条件的稳定性而言,ISSR优于RAPD,且总的来说ISSR能检测到比RAPD更多的遗传变异。另外,本研究推断人类活动的干扰和生境的片断化是导致伯乐树濒危现状的主要因素之一。
To provide biological foundation for the utilization and breeding of Chinese Bretschneidera germplasm,ISSR and RAPD were applied to detect genetic diversity of 23 accessions of Chinese Bretschneidera germplasm.79 bands were applied with 13 ISSR primers,of which 57（63.29%）were polymorphic.57 bands were applied with 10 RAPD primers,of which 33（57.89%）were polymorphic.Effective number of alleles Ne,Nei′s index H,the Shannon information index were 1.4306 and 1.3601,0.2305 and 0.2215,0.3405 and 0.3145.The two molecular markers demonstrate that both ISSR and RAPD are efficient approaches for genetic diversity analysis of Chinese Bretschneidera germplasm and ISSR is more suitable comparatively in terms of reproducibility and ability of detecting genetic polymorphism.The influence of human activity and forest fragmentation may play a main role in creating this species′s current endangered status.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2011年第3期362-367,共6页
Journal of Plant Genetic Resources
基金
浙江省重大科技专项(2006C12059-4)
浙江省科技重点项目(2006C22064)
关键词
伯乐树
RAPD
ISSR
遗传多样性
Bretschneidera sinensis Hemsl.
RAPD
ISSR
Genetic diversity
