摘要
目的: 分离伯氏疟原虫敏感株( C S) 与抗氯喹株的氯喹(chloroquine C Q) 结合蛋白。方法: 给两株感染鼠ig C Q400 mg/kg , 3 h 后, 收集伯氏疟原虫用( Ultrogel R) Ac A34 凝胶柱分离蛋白, 按 Bergqvist 法抽提与蛋白结合的 C Q, 然后用 H P L C ( 外标法) 定量。结果: C S 有58 个蛋白峰, 均与 C Q 结合, 其中以 No64 ~86部分( 峰9) 结合的 C Q 量最多, 占总结合量的164 % 。抗氯喹伯氏疟原虫( C R) 有40 个蛋白峰, 除8 个峰外,均与 C Q 结合, 结合量最多的蛋白部分与 C S相同, 在 No60 ~83 部分( 峰6 及峰7) , 但是它与 C Q 的结合能力( C Qnmol/ mg 蛋白) 显著高于 C S。结论: 分离与测定了 C S和 C R 两株疟原虫各个蛋白峰的分布与定量, 及其与 C Q 结合的能力。在同一株疟原虫中, 各蛋白与 C Q 的结合能力存有差异, 在 C S与 C R 间此差异更大。
AIM: To isolate and quantitate chloroquine (CQ)\|binding proteins in both CQ\|sensitive (CS) AND CQ\|resistant (CR) Plasmodium berghei . METHODS: P\^berghei infected mice were each given ig CQ 400 mg/kg, respectively, 3 h later, the proteins of the parasites collected were separated using Ultrogel R AcA 34 gel column , followed by CQ extraction , the CQ concentration was determined by HPLC (extra standard). RESULTS: There were 58 protein peaks in the CS, all of which were bound with CQ,fraction Nos. 64~86 (peak 9) being the predominant, accounting for 16\^4% of the total amount of CQ\|binding protein. In the CR there were 40 protein peaks, among which 32 were bound with CQ, the predominant fraction Nos\^60~83 (peaks 6,7),had a higher CQ\|binding ability than CS. CONCLUSION: The distribution and amount of each CQ\|binding protein peak in both CS and CR strains of P.berghei were demonstrated, exhibiting differences in the ability of CQ\|binding not only between CS and CR strains but also within the same strain.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1999年第4期228-230,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金