摘要
目的:研究胃癌细胞来源的Exosome对同源肿瘤细胞增殖的影响,初步探讨PI3K/Akt和MAPK/ERK信号转导通路在此过程中的作用.方法:采用离心超滤和蔗糖密度梯度离心的方法从胃癌MGC803细胞的上清液中分离出胃癌细胞来源的Exosome.应用透射电子显微镜观察Exosome的形态,MTT检测细胞活力,Western blot检测蛋白的表达.结果:透射电子显微镜下观察胃癌MGC803细胞来源的Exosome具有特征性的盘状结构,由双层膜构成,他们的直径在30-100nm.Westernblot结果显示Exosome表面富含CD9和TSG101分子.Exosome能以时间和剂量依赖性的方式促进MGC803和其同源肿瘤细胞SGC7901细胞的增殖,在此过程中伴随有磷酸化Akt和磷酸化ERK表达的上调.结论:胃癌细胞来源的Exosome能促进同源肿瘤细胞的增殖,其机制可能与激活PI3K/Akt和MAPK/ERK信号转导通路有关.
AIM：To investigate the effect of gastric cancer exosomes on homologous tumor cell prolifera-tion and to evaluate the role of PI3K/Akt and MAPK/ERK pathways in this process.METHODS：Exosomes were isolated and purified from human gastric cancer MGC803 cells by serial centrifugation and sucrose gradient ultracentrifugation and observed by electron microscopy.Cell proliferation was measured by MTT assay,and protein expression was assayed by Western blot.RESULTS：Gastric cancer exosomes had a characteristic saucer-like shape that was limited by a lipid bilayer,and their diameter ranged from 30 to 100 nm.CD9 and TSG101 were abundantly distributed on the surface of exosomes.Gastric cancer exosomes significantly increased MGC803 and SGC7901 cell proliferation in a time-and dose-dependent manner.Treatment with exosomes up-regulated the expression of phosphorylated Akt and ERK in a time-depen-dent manner.CONCLUSION：Gastric cancer exosomes promote homologous tumor cell proliferation possibly by activating the PI3K/Akt and MAPK/ERK pathways.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第11期1109-1114,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.31000607~~
