摘要
目的探讨外周血CD4+CD25+T淋巴细胞与支气管哮喘的关系。方法用流式细胞术分别检测支气管哮喘患者34例(A组)和健康人25例(B组)外周血CD4+CD25+T淋巴细胞的百分率,采用ELISA法检测外周血血清白细胞介素10(IL-10)水平,用化学发光法检测外周血IgE水平,并分析CD4+CD25+T淋巴细胞与外周血IgE及IL-10水平的相关性。结果 A组外周血CD4+CD25+T淋巴细胞百分率显著低于B组[(5.90±1.10)%vs.(7.13±1.48)%](P<0.05),且与血IL-10呈正相关,与IgE呈负相关(P<0.05)。结论 CD4+CD25+T淋巴细胞在支气管哮喘患者中高表达,与IL-10及IgE有一定的相关性,是参与支气管哮喘的发生、发展的重要T淋巴细胞。
Objective To explore the correlation between the expression of CD4＋CD25＋T lymphocyte and asthma.Methods The percentages of CD4＋CD25＋T lymphocyte of peripheral blood were detected with flow cytometry in 34 asthma patients（group A） and 25 healthy controls（group B）.Plasma IgE was tested by chemiluminescence and serum interleukin-10（IL-10） was detected by ELISA.Results The expression of CD4＋CD25＋T lymphocyte was significantly lower in group A than that in group B [（5.90±1.10）% vs.（7.13±1.48）%]（P0.05）,which was positively correlated with serum IL-10 and negatively correlated with plasma IgE（P0.05）.Conclusion CD4＋CD25＋T lymphocyte was overexpressed and associated with serum IL-10 and plasma IgE in patients with asthma,which suggests that CD4＋CD25＋T lymphocyte may play a role in the development of asthma.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第10期1171-1172,共2页
Jiangsu Medical Journal
