摘要
【摘要】目的观察糖尿病视网膜病变（DR）对循环外周血内皮祖细胞（EPCs）的影响。方法雄性成年Wistar大鼠60只，随机分为正常对照组和糖尿病组。后者通过腹腔注射链脲佐菌素（STZ）建立DR模型。采用流式细胞仪分别计数建模后1周、l、3、6个月各组大鼠循环外周血EPCs数量，同时于各相应时间点摘除大鼠眼球行苏木精伊红（HE）染色、视网膜血管消化铺片过碘酸一Schiff（PAS）染色，通过光学显微镜以及透射电子显微镜进行视网膜组织病理学检查。对比分析EPCs数量以及EPCs数量与DR形态学变化的相互关系。结果大鼠注射STZ1周、1、3、6个月后循环外周血EPCs数量分别为（25±7）、（28±8）、（39±7）、（43±7）个／20万个单个核细胞，对照组为（45土4）个／Z0万个单个核细胞。与正常对照组大鼠相比，糖尿病组EPCs数量均降低（F=8．933，P〈0．01）。随着观察时间的延长，糖尿病组EPCs数量逐渐回升，而视网膜结构、光感受器细胞、血管也逐渐出现病理性改变。光学显微镜下，与正常对照组比较，大鼠注射STZ后1周和1个月组视网膜各层结构清晰完整，但厚度开始变薄，1个月组视网膜细胞排列紊乱，细胞核肿胀、体积增大，光感受器细胞减少，随病程时间增加这些改变逐渐加重，3、6个月组视网膜厚度更薄，细胞排列紊乱更加明显，部分扩张的血管有向外突出、破溃的趋势。透射电子显微镜下，毛细血管内皮细胞水肿，线粒体肿胀；毛细血管基底膜增厚，管腔狭窄，6个月组甚至出现管腔闭塞和视网膜微动脉瘤；光感受器细胞膜盘间隙扩大，排列紊乱，线粒体水肿，细胞早期空泡变性。结论糖尿病大鼠外周血EPCs数量较正常大鼠降低。随着DR的进展，EPCs数量逐渐回升。
Objective To observe the effect of diabetic retinopathy on endothelial progenitor cells （EPCs） from peripheral blood. Methods Sixty male Wistar rats were divided into control group and diabetes group. The rats in diabetes group were induced with streptozotoein （STZ） injection for diabetic retinopathy model. Flow cytometry was used to identify and count the number of EPCs from peripheral blood at 1 week, 1, 3 and 6 months after injection. All eyeballs were examined by hematoxylin and eosin （HE） staining, periodic acid Sehiff＇s （PAS） staining of trypsin digested retinal vessels flat preparation and transmission electron microscope. EPCs count, and the relationship between DR morphological changes and EPCs count were compared and analyzed. Results The quantity of EPCs from peripheral blood at 1 week, 1, 3 and 6 months after STZ injection were 25±7, 28±8, 394±7, 43±7 cells per 200 000 monocytes respectively, which decreased compared with the control group 45 ±4 cells per 200 000 monocytes （F= 8. 933,P〈0.01）. The quantity of EPCs was gradually increased at 1 week, 1, 3 and 6 months after STZ injection, accompanied with responsive pathological changes of retinal structure and vessels. The thickness of retina at 1 week and 1 month after injection were reduced slightly. The number of retinal ganglion cells reduced, with the time passing by. Endothelial cells were edema, mitochondrial was swollen, capillary basement membrane was thicken, lumen was significant stenosis, lumen occlusion and retinal artery aneurysm were observed at 6 months after STZ injection. Conclusion The number of EPCs increases graduallv throughout the development of DR.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2011年第3期250-254,共5页
Chinese Journal of Ocular Fundus Diseases
基金
天津市应用基础与前沿技术研究计划（10JCZDJC20300）
关键词
糖尿病视网膜病变/病理生理学
内皮
血管
造血干细胞
糖尿病
实验性
Diabetic retinopathy/pathophysiology
Endothelium,vascular
Myeloid progenitorcells
Diabetes mellitus,experimental
