摘要
目的:建立泡囊草中不同部位总生物碱的含量测定方法。方法:以氢溴酸东莨菪碱为对照品,用酸性染料溴甲酚绿染色,采用分光光度法,在415nm波长下测定样品中总生物碱的含量。结果:吸光度值与生物碱含量线性关系良好(r=0.9998);回收率为98.68%,RSD为2.19%;泡囊草叶生物碱含量最高,达到5.6695mg/g,果、茎其次,根中最低。结论:泡囊草部位不同总生物碱含量也不同,该测定方法简便可靠,可用于泡囊草质量控制。
Objective： To establish a method for determination of total alkaloids from different parts of Physochlaina Physaloideslis saxicola.Methods：Using scopolamine hydrobromide as control sample.The contents of total alkaloids in the samples were tested at 415 nm with the spectroscopy by the means of acid dye method.Results：The calibration curve of berberine were linear（r=0.9998）,and the average recovery was 98.68%,RSD was 2.19%.The content of alkaloids in leaves the highest,with 5.6695 mg/g,followed by fruits and stems / roots.Conclusion： This method is simple and effective,and the contents of total alkaloids were different among different parts of Physochlaina Physaloideslis saxicola.
出处
《内蒙古医学院学报》
2011年第1期46-49,共4页
Acta Academiae Medicinae Neimongol
基金
内蒙古自然科学基金资助项目(2009BS1230)
关键词
泡囊草
总生物碱
分光光度法
氢溴酸东莨菪碱
physochlaina Physaloideslis saxicola
total alkaloids
spectroscopy
scopolamine hydrobromide
