摘要
目的观察白细胞介素1β（IL-1β）干预人牙龈成纤维细胞中肿瘤坏死因子受体相关因子6（TRAF6）表达水平,为研究细胞因子对牙周组织的调控作用提供理论依据。方法采用原代培养的人牙龈成纤维细胞,取3-8代细胞以不同浓度IL-1β进行干预,24 h后观察干预后人牙龈成纤维细胞形态学变化,采用免疫组织化学方法检测TRAF6在各组细胞中的表达。结果 IL-1β干预后,人牙龈成纤维细胞形态改变不明显;免疫组化结果显示,以0.1、0.5、1、5、10 ng/mL的IL-1β干预后,细胞中TRAF6呈阳性表达,并且随IL-1β干预浓度升高而增强,TRAF6蛋白表达量吸光度分别为（0.15±0.012）、（0.19±0.010）、（0.20±0.008）、（0.24±0.010）、（0.27±0.011）。两两比较,差异均有统计学意义（均P〈0.01）。结论 TRAF6表达水平变化提示,TRAF6可能是参与牙龈成纤维细胞功能调控的重要信号分子。
Objective To examine the expression of tumor necrosis factor receptor-associated factor-6（TRAF6）in human gingival fibroblasts（HGFs） by the interference of interleukin-1β（IL-1β）,and to provide new document to the research of the effect of cytokine network on periodontal ligament tissue.Methods HGFs were isolated and cultured.The cells from the third to eighth generation were cultured and interfered with various concentrations of IL-1β.The morphology changes of HGFs were observed after 24 hours and the expression of TRAF6 in every experimental group was detected with immunohistochemical method.Results The morphology change of HGFs was not obvious with the increase of IL-1β concentration.The immunohistochemistry analyses showed that there was a positive expression of TRAF6 in HGFs with IL-1β intervention and the expression increased with the increment of IL-1β concentration.Conclusion The results suggest that TRAF6 may be an important signal factor which participates in the regulation of HGFs＇ function and periodontitis.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第6期721-723,共3页
Chinese Journal of Public Health
基金
山西省自然科学基金(20090311057-6)
