摘要
目的:建立测定盐酸二甲双胍缓释片中盐酸二甲双胍含量的方法。方法:采用高效液相色谱法。色谱柱为HypersilODS2柱为,2流0μ动L相,柱为温0为.053%0庚℃烷。磺结酸果钠:盐溶酸液二(用甲磷双酸胍溶检液测调浓节度p在H0至.114～.0)1-0乙.7腈0(μg8·4m∶1L6-)(1,r检=测0.波99长97为)范23围3内nm与,流峰速面为积1积.0分m值L·呈mi良n-好1,线进性样量关系;平均加样回收率为98.17%、99.69%、100.19%,RSD=0.60%。结论:本方法简便、准确,可用于二甲双胍缓释片的质量控制。
OBJECTIVE： To establish the method for the content determination of main component of Metformin hydrochloride sustained- release tablets. METHODS：HPLC was conducted on a Hypersil ODS2 with mobile phase consisted of 0.05% sodium heptanesulfonate solution （pH adjusted to 4.0）-acetonitrile （84 ∶ 16） at a flow rate of 1.0 mL·min-1. The UV detection wavelength was set at 233 nm. The injection volume was 20 μL and the column temperature was 30℃. RESULTS： The linear range of metformin hydrochloride was 0.11～10.70 μg·mL-1 （r=0.999 7） with an average recovery of 98.17%, 99.69%, 100.19%（RSD=0.60%）. CONCLUSION： The method is simple and accurate for the content determination of Metformin hydrochloride sustained-release tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第24期2296-2297,共2页
China Pharmacy
关键词
高效液相色谱法
盐酸二甲双胍缓释片
含量测定
HPLC
Metformin hydrochloride sustained-release tablets
Content determination
