摘要
目的 旨在探讨肌肉注射成纤维细胞生长因子受体(FGFR)活化抑制剂———5’甲基硫代腺苷(5’MTA)对大鼠体内反应性星形胶质化的调控作用。方法 Wistar大鼠20只均分为2大组:甲组损伤后4d处死(含5只加药鼠),乙组损伤后8d处死(含5只加药鼠);两组中非加药鼠作相应单纯损伤对照组。在冰冻和石蜡切片上行HE染色和免疫组织化学染色观测碱性成纤维细胞生长因子(bFGF)和胶质纤维酸性蛋白(GFAP)的表达并作形态定量分析。结果 5’MTA加药组在伤后4d其伤道旁细胞数量以及bFGF和GFAP的反应强度均明显低于对照组;加药组伤道宽度(8d时)明显大于对照组,提示其愈合受到5’MTA阻抑。
Purpose To determine the interference of 5' methylthioadenosine (5'?MTA),an inhibitor of fibroblast growth factor receptor (FGFR),with the reactive astrogliosis of injured rat brain. Methods Twenty Wistar rats (male,200-250 g) were divided into 2 groups (average 10).Five rats of one group had brain injured and admi nistered 5'?MTA for 3 days,while 5 rats of the other group with the same insult had drug for 7 days.The other 10 rats of the two groups only received brain injury as control HE,immunostain of bFGF and GFAP was used and determined with morphometry.Results 5'?MTA treatment decreased the cell number and weakened the reaction of bFGF and GFAP in the areas adjacent to the injury compared with those of the control at 4 th day;eight days later,the lesion width interfered with 5'?MTA was apparently wider than that of the control. Results 5'?MTA treatment decreased the cell number and weakened the reaction of bFGF and GFAP in the areas adjacent to the injury compared with those of the control at 4 th day;eight days later,the lesion width interfered with 5'?MTA was apparently wider than that of the control. Conclusions Systemic administration of 5'?MTA may partly inhibit the rat reactive astrogliosis.
出处
《上海医科大学学报》
CSCD
1999年第5期318-320,共3页
Journal of Fudan University(Medical Science)
基金
国家自然科学基金!资助(39470 2 87)
