摘要
目的 探讨骨桥蛋白（OPN）及核因子-κB（NF-κB）与狼疮性肾炎（LN）患者临床及病理之间的关系.方法 应用免疫组化方法检测56例LN患者及10例对照组肾组织中OPN及NF-κB的表达,并与临床病理指标进行综合分析.结果 OPN及NF-κB在LN肾组织表达率高于对照组,以Ⅳ型显著,肾组织中OPN的表达量与血肌酐、24小时尿蛋白定量、狼疮性肾炎疾病活动指数、病理活动性指数呈正相关（P＜0.05）,但与血清C3、病理慢性指数无明显相关性;NF-κB与Scr、病理活动性指数、病理慢性指数、狼疮性肾炎疾病活动指数、24小时尿蛋白定量呈正相关（P＜0.05）.OPN及NF-κB在LN肾组织中的表达呈显著正相关（P＜0.01）.结论 LN肾组织中OPN及NF-κB的异常表达与肾脏病理改变及肾损害临床指标密切相关,其表达量与肾损害严重程度一致,且OPN和NF-κB具有协同作用,在LN发病机制中可能起重要作用.
Objective To investigate the clinicopathological significance of osteopontin （OPN） and nuclear factor-KB （NF-KB） expression in lupus nephritis （LN）. Methods Immunohistochemistry was used to detect the expression of OPN and NF-KB in renal tissue of 56 cases with LN and 10 cases in the normal control group and the relationships of their expression with the clinicopathological parameters of lupus nephritis were analyzed. Results Compared with the control group, the situ expression of OPN and NF-κB in the lupus nephritis was significantly higher,especially in IV-type LN. Correlation analysis indicated that OPN was correlated significantly with the level of serum creatinine（Scr） ,24 hour Urinary Protein（ 24 h UP）, SLE disease activity index （ SLEDAI ） points and the scores of activity index （AI） （ P 〈 0.05） ,while no correlation was among OPN,the level of serum C3 and the score of chronic index（CI）. NF-κB was correlated with the level of Scr,the scores of AI and CI,SLEDAI points, as well as 24 h UP（P 〈 0.05 ）, and it was closely related to OPN expression （P 〈 0.01 ）. Conclusion Abnormal expression of OPN and NF-κB in renal tissue of LN was related with renal pathology and clinical index for renal damage. This two two factors have a strong synergism and might play critical roles in the pathogenesis of LN.
出处
《临床内科杂志》
CAS
2011年第5期340-343,共4页
Journal of Clinical Internal Medicine
基金
安徽省自然科学研究项目（KJ20078276）
