摘要
以MS为基本培养基,在不同的激素成份下,培养口红花(AeschynanthuspulcherG.Don)叶片,得出适合诱导其愈伤组织培养基为MS+2,4-D0-4mg·L-1+BA0-3mg·L-1,诱导率为60%,适合分化不定芽的培养基为BA0-5mg·L-1和NAA0-3mg·L-1,其分化芽可达3～4倍。采用γ射线辐射其幼芽,辐射剂量为1-72Gy·min-1,总剂量为40～45Gy,诱变率达6%,同时对其壮苗进行生根培养,选用1/2MS基本培养基附加IBA0-3mg·L-1和NAA0-5～0-6mg·L-1,生根率100%,生根时间40d。
The leaf blades of Aeschynanthus pulchira were cultured by means of different hormone compositions,taking Ms as the basic culture medium.It was obtained that the suitable culture medium of inducing their callus was Ms+2,4-D0 4mg·l -1 +BA0 3mg·l -1 and the induction rate was 60%.The suitable culture medium of differentiating adventitious buds was BA0 5mg·l -1 and NAA0 3mg·l -1 and its differentiated buds could reach 3～4times.The V-ray was adopted to radiate the juvenile buds,the radiation dosage was 1 72Gy·min -1 ,the total dosage was 40～45 Gy and the mutagensis rate reached 6%.Meanwhile the rooting culture was conducted for the sturdy Plants,the 1/2Ms basic culture medium was selected with the addition of the IBA0 3mg·l -1 & NAA0 5～0 6mg·l -1 ,the rooting rate was 100% and the rooting time was 40days.
出处
《福建林业科技》
北大核心
1999年第4期18-21,共4页
Journal of Fujian Forestry Science and Technology
基金
福建省自然科学基金
关键词
口红花
愈伤组织
细胞诱变
Aeschynanthus pulchira
Callus
Cell mutagensis
