摘要
本试验采用石蜡切片技术和番红-固绿染色法对矮生和蔓生菜豆的叶片进行了解剖学研究,为选育优良的矮生菜豆品种提供理论依据。结果表明:矮生菜豆叶片下表皮的气孔数大于蔓生品种,且差异极显著。矮生品种叶片厚度、海绵组织厚度、海绵组织厚度/叶片厚度明显小于蔓生品种;而栅栏组织厚度/叶片厚度、栅栏组织厚度/海绵组织厚度则大于蔓生品种,叶片主脉维管束木质部导管数目、输水面积、韧皮部截面积均表现出矮生品种小于蔓生品种。因此,叶片的栅栏组织厚度/海绵组织厚度可以用于矮化菜豆的形态指标筛选。
In this experiment,we used paraffin sections technology and safranine-fast green dyeing to study leaf anatomical structure of dwarf and vine bean （Phaseolus vulgaris）,and it can provide theoretical basis for selecting and cultivating superordinary dwarf bean species.The results showed that the number of spiracles in leaf lower epidermis of dwarf bean were more than that of vine bean,and the disparity was very significant.The leaf blade thickness,spongy tissue thickness and the rarefaction extent of leaf blade organizational structure of dwarf bean were fewer than that of vine bean.But the tightness of leaf blade organizational structure and palisade tissue thickness/spongy tissue thickness of dwarf bean were more than of that of vine bean.The number of catheters in main vein bundle,the area of water delivery and the section area of phloem in the main nerve of leaf of dwarf bean were fewer than that of vine bean.Therefore,palisade tissue thickness/spongy tissue thickness could be used as the morphology screening indicator of dwarf bean.
出处
《中国农学通报》
CSCD
北大核心
2011年第13期199-203,共5页
Chinese Agricultural Science Bulletin
基金
黑龙江省自然科学基金(230340)
高等学校博士点专项科研基金(200802240008)
