摘要
目的:采用一测多评法同步测定中成药香连制剂中4个生物碱的含量,探讨一测多评法在中成药中的适用性。方法:以香连制剂为研究对象,通过建立小檗碱与表小檗碱、黄连碱、巴马汀的相对校正因子,并将该校正因子应用于上述4种生物碱的含量测定,实现一测多评;同时采用外标法测定4种生物碱的含量,比较计算值与实测值的差异。结果:16批不同批次的香连制剂中4种生物碱的含量可以用一测多评法进行测定,其计算值与实测值间无显著差异。结论:以表小檗碱、黄连碱、巴马汀、小檗碱为指标,采用一测多评法控制香连制剂的质量是可行的。
Objective：To examine the feasibilities of the method,quantitative analysis of multi-components by single marker（QAMS） for determination of four alkaloids in Xianglian preparations.Method：A HPLC method was developed to determine the contents of four alkaloids,epiberberine,coptisine,palmatine and berberine in Xianglian preparations.Berberine was selected as the internal reference substance,and 3 relative correction factors to berberine were calculated.The contents of the four alkaloids in 16 batches of samples were determined by both external standard method and QAMS.The new method was evaluated by comparing the quantitative results with of the external standard method.Result：No significant differences were found between the quantitative results of four alkaloids in 16 batches of samples determined by the two methods.Conclusion：QAMS is feasible for determination of four alkaloids simultaneously,and the developed method can be used for quality control of Xiang Lian preparations.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第11期1445-1448,共4页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09308-003
2009ZX09301-005
2009ZX09502-021)
中医药行业科技专业项目(200707009)
关键词
一测多评法
相对校正因子
HPLC
香连制剂
quantitative analysis of multi-components by single-marker（QAMS）
relative correction factor（RCF）
HPLC
Xianglian preparations
