摘要
目的:研究白及多糖对糖尿病溃疡愈合的治疗作用及其作用机制。方法:SD大鼠56只,糖尿病模型通过腹腔注射链脲佐菌素(STZ,50 mg.kg-1)建立,72 h后,血糖大于≥16.7 mmol.L-1即认为糖尿病造模成功。4周后,切割2个直径为1.8cm大小圆的全层皮肤,糖尿病溃疡模型制造后,2个创面分别用白及多糖和对照PBS干预;于伤后3,7,14,21d时相点对创面愈合面积、组织形态学改变、成纤维细胞(FB)增殖情况、羟脯氨酸(OHP)含量、髓过氧化物酶(MPO)含量进行观察与检测。结果:白及多糖能显著促进糖尿病溃疡创面愈合(与PBS组比较P<0.05),病理组织学检查发现白及多糖能有效刺激炎症细胞浸润,促进上皮组织形成,FB增殖明显,OHP含量增加。结论:白及多糖可能通过促进炎症细胞浸润、增加OHP含量的合成和释放、FB的增殖,从而促进糖尿病溃疡创面愈合。
The aim of this study was to evaluate the efficacy of Bletilla striata polysaccharide on diabetes mellitus ulcers.Diabetes mellitus animal model was established by single ip injection of streptozotocin（STZ,50 mg·kg-1） with the criteria of blood glucose≥16.7 mmol·L-1 after 72 h.4 weeks after STZ injection,each animal received two full thickness incisional wounds（1.8 cm in diameter）.The wounds then were divided into B.striata polysaccharide group and PBS group.Wound closure rate,fibroblast（FB） infiltration,hydroxyproline（OHP） content and myeloperoxidase（MPO） levels were examined on day 3,7,14,21 post wound.The treatment of B.striata polysaccharide significantly facilitated diabetes mellitus ulcers healing compared to PBS group.Histological analysis showed that B.striata polysaccharide markedly increased inflammatory cell infiltration in wound area.The herb also strongly evaluation of FB,OHP demonstrated a significantly increased in B.striata polysaccharide group.B.striata polysaccharide group promoted wound closure by means of enhanced inflammatory cell infiltration and re-epithelialization,and the promotion of FB and OHP levels.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第11期1487-1491,共5页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09311-003)
上海市教委高校一氧化氮与炎症医学E研究院计划项目(E-04010)
上海市科委基础研究重点项目(08DZ1972104)
关键词
糖尿病溃疡
白及多糖
diabetes mellitus ulcers
Bletilla striata polysaccharide
