摘要
目的观察新型铁螫合剂CHGN2957对大鼠肾缺血再灌注损伤（I／R）急性期的保护作用。方法选用雄性SD大鼠90只，随机分成5组（n=18）：假手术组、CHGN2957高剂量组、低剂量组、溶剂组和阳性对照组。建成大鼠急性。肾I／R模型。药物干预均从手术前2d开始，直至术后12d结束。观察大鼠死亡率、体质量变化，测定肾功能和肾组织中超氧化物歧化酶（SOD）活力、谷胱甘肽（GSH）还原酶活力和丙二醛（MDA）含量并进行肾组织病理切片观察。结果术后第3天CHGN2957溶剂组的体质量（242．1±16．2）g、SOD活力（1．23±0．13）U／mg、GSH还原酶活力（336±15）u／L明显低于其他各组（P〈0．05），而血清尿素氮（62．3±3．1）mmol／L、血清肌酐（310．00±21．02）μmol／L、MDA含量（186．5±16．7）nmol／mg明显高于其他各组（P〈0．05）。肾组织病理评分显示CHGN2957溶剂组的肾小管损伤明显重于其他各组（P〈0．05）。结论CHGN2957作为一种新型铁罄合剂．在大鼠急性肾L／R过程中能有效减轻大鼠肾I／R．并对肾起保护作用。
Objective To observe the protecgive effects of CHGN2957 on acute renal ischemia/ reperfusion injury （I/R） model in rats. Methods Ninety Sprague-Dawley male rats were randomly divided into five groups （n= 18 each）： sham-operative group, high-dose CHGN2957 group, low-dose CHGN2957 group, CHGN2957 vehicle group and positive group. The administration lasted from two days before operation to twelve days after operation. After seccessful establishment of the model, mortality, weight changes, the renal function, superoxide dismutase （SOD） activity, glutathione （GSH） reductases activity and malondialdehyde （MDA） content in renal tissue were recorded, and the pathological changes were observed. Results Weight （242. 1 ± 16. 2） g, SOD activity （ 1.23 ± 0. 13） U/mg and GSH reduc- tases activity （336 ± 15 U/L） in CHGN2957 vehicle group were reduced significantly as compared with other groups three days after I/R （ P 〈 0.05 ）, but SUN （ 62. 3 ± 3.1 ） mmo]/L, SCr （ 310.00 ± 21.02 ） μmol/L, MDA content （ 186. 5 ± 16. 7 ） nmol/mg were higher than others obviously （ P 〈 0.05 ）. Pathological observation showed renal tubular injury was more serious in CHGN2957 vehicle group （ P 〈 0. 05 ）. Conclusion CHGN2957 as a new iron chelator was effective to ameliorate renal I/R in rats.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第6期861-864,共4页
Chinese Journal of Experimental Surgery
关键词
肾
缺血
再灌注损伤
铁螯合剂
Kidney
Isehemia
Reperfusion injury
Iron chelator
