摘要
目的建立大鼠肾冷缺血再灌注损伤（IRI）的模型。方法封闭群sD大鼠24只，随机分为2组（n=12）：A组（对照组），B组（实验组）。A组切除右肾并游离左肾蒂，60min后关闭腹腔切口。B组采用冷缺血再灌注模型，主要步骤：（1）冷灌注：右肾动脉插管对左肾原位灌注。通过右肾静脉插管将灌注液引流出体外，完成冷灌注后切除右肾，阻断左。肾蒂。（2）冷缺血保存：将已充分游离的左肾牵至腹腔外，在自制保存袋中冷保存。（3）再灌注：60min后，去除保存袋，开放血流，再灌注左肾，左肾复位，缝合切口；2组大鼠均在术后24h再次手术切除左肾。肾组织进行光镜、电镜形态学检查，检测肾组织匀浆中超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量，术前与术后24h取血标本进行测定血尿素氮（BUN）、肌酐（Cr）评估肾功能。结果（1）形态学检查（光镜与电镜超微结构）：A组肾脏组织形态结构正常，B组损伤表现明显；（2）A组手术前后比较血浆BUN、Cr测定值差异均无统计学意义（P〉0．05）。IR后的B组均高于术前，差异有统计学意义（P〈0．05）；（3）IRI后A组肾组织匀浆SOD活力高于B组（P〈0．05），A组肾组织匀浆MDA含量测定值低于B组，差异有统计学意义（P〈0．05）。结论建立的模型要求条件简单、易行，可用于肾移植冷缺血再灌注损伤相关的研究；
Objective In this study, for studying IRI in kidney transplantation. , we established the models of cold ischemia and reperfusion injury in rats. Methods Twenty four SD rats were randomly assigned to two groups：control （A）, and experimental （B） group. Group A was only removed the right kid- ney. Cold ischemia reperfusion was performed as the follow-listed model in Group B. The main process of the model： （ 1 ） Perfusing left kidney ： after resected the right kidney of the rat, one pipe was put in the re- mainder right renal artery to perfuse the left kidney. The perfusion flowed out through another pipe in the right renal vein. The blood vessels of left kidney were clipped after cold perfusion. （2） Cold ischemic conservancy：the operation table was leant to left side, and the left kidney was taken out of abdominal cavity then stored in a cold bag which was full of ice and water,but the vessels of that were intact. （3） Reperfus- ing left kidney：after 60 minutes, the clip was removed. Left kidneys of all rats in two groups were removed to be detected. Structure of the kidney was evaluated by light microscopy and electronic microscopy. Superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in the renal tissues was examined, and the renal function was also assessed by determining the levels of blood urea nitrogen （BUN） and serum creatinine （CR） before and 24 hours after operation. Results （ 1 ） Morphologic change （ hematoxylin-eosin staining） ：A normal morphology was observed by light microscopy and electon microscopy in group A. Significant injury was detected in group B. （2）In group A, there was not significant difference about BUN and CR between before and after operation （ P 〉 O. 05 ） , but in Group B, those increased significantly at 24 hour after operation （P 〈 0. 05 ）. （3） Activity of SOD in renal tissues in group A was higher than those in group B （ P 〈 0. 05 ） , meanwhile, Content of MDA in group A was lower than those in group B （ P 〈 0. 05 ）. Conclusion The rat renal cold ischemia reperfusion model we established is feasible regardless of experimental conditions, and can be studied as the events following IRI in kidney transplantation.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第6期998-1000,共3页
Chinese Journal of Experimental Surgery
关键词
肾
冷
缺血
再灌注损伤
Kidney
Cold
Ischemia
Reperfusion injury
