摘要
目的 选用前列腺素合成抑制剂布洛芬（Ｉｂｕ）对创伤后细胞免疫功能变化的作用。方法 Ｂａｌｂ／ｃ小鼠行创伤性截肢手术后分Ｉｂｕ 组（腹腔注射Ｉｂｕ １ｍ ｇ／ｋｇ，ｎ＝ ２８）和生理盐水组（腹腔注射生理盐水０．５ｍ ｌ，ｎ＝ ２８），正常小鼠作为对照组。于术后１、３、５、７ 天分别每组取７ 只小鼠测其淋巴细胞增殖功能和自然杀伤细胞（ＮＫ 细胞）活性。结果 创伤后小鼠脾脏淋巴细胞增殖功能明显下降（Ｐ＜ ０．０１），于第７ 天尚未恢复（Ｐ＜ ０．０５）；Ｉｂｕ 组小鼠在创伤后也有明显抑制（第１ 天Ｐ＜ ０．０１，第３ 天Ｐ＜ ０．０５），第５天开始恢复（Ｐ＞ ０．０５），而与生理盐水组比，创伤后第１ 天即呈显著增高（Ｐ＜ ０．０１）。创伤后给Ｉｂｕ 小鼠的的ＮＫ 细胞杀伤活性也有提高，虽较对照组明显降低（Ｐ＜ ０．０１），但第１、３ 天均较生理盐水组明显提高（Ｐ＜ ０．０５ 或０．０１）。结论 布洛芬可部分提高创伤后小鼠受抑制的细胞免疫功能。
Objective To evaluate the effect of cyclooxygenase inhibitor ibuprofen,on the cellular immunity of mice after trauma.Methods The study was conducted in Balb/c mice(6 8weeks old)with one of their legs amputated.After the operation,the mice were allocated to receiving ibuprofen 1mg/kg ip(Ibu group) or normal saline(NS group).The normal mice served as control(control group).Then 1,3,5,7days after the operation,seven mice of each group were sacrificed to assess their splenocyte proliferation and NK cell cytotoxicity.Results Splenocyte proliferation was depressed markedly in the NS group(P<0.01 vs control group) and had not recovered 7 days later (P<0.05 vs control group).Splenocyte proliferation above in Ibu group was much lower than control group during the 1st and 3rd day (P<0.01),but recovered on the 5th day(P>0.05) and was higher than NS group(P<0.01).The percent of NK cell cytotoxicity of Ibu group was also higher than of NS group(1st day P<0.01,3rd day P<0.05),lower than of control group (P<0.01).Conclusion The depressed cellular immunity following trauma can be partially reversed by cyclooxygenase inhibitor,indicating that prostaglandin may play an important role in the trauma induced immunosuppression.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
1999年第8期477-479,共3页
Chinese Journal of Anesthesiology
