摘要
目的探讨当归注射液对大鼠慢性肺动脉高压的影响。方法30只雄性Sprague-Dawley大鼠随机分成对照组、低氧组、当归保护组，每组10只。当归保护组每天缺氧前腹腔注射25％当归注射液[10me,／（kg·d）]；对照组、低氧组于每天缺氧前腹腔注射等量生理盐水。常压低氧建立大鼠肺动脉高压模型，4周末测各组大鼠平均肺动脉压（mPAP）、应用免疫组织化学法和图像分析技术定量检测大鼠肺小动脉管壁和内皮细胞增殖细胞核抗原（proliferating cell nucle arantigen，PCNA）、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）的表达水平；同时肺小动脉行HE染色，测定肺小动脉管壁面积占肺小动脉总面积的百分比（wA％）。结果对照组、低氧组、当归保护组的mPAP分别为10．50±1．90、35．36±9．11、18．32±2．30（nllnHg）；wA％分别为52．71±5．16、82．38±8．43、64．58±9．54（％），低氧组的mPAP、wA％均高于对照组（P〈0．01），而当归保护组mPAP、wA％均明显低于低氧组（p〈0．01）。对照组、低氧组、当归保护组肺小动脉PCNA表达水平分别为3．15±1．10、24．50±5．72、12．67±3．46（％），缺氧组显著高于对照组（P〈0．01），而当归保护组明显低于低氧组（P〈0．01）。对照组、低氧组、当归保护组肺小动脉血管内皮iNOS蛋白表达水平分别为2．13±1．01、17．33±3．53、37．50±7．04（％），低氧组显著高于对照组（P〈0．01），而当归保护组明显高于低氧组和对照组（P〈0．01）。结论当归注射液可以降低慢性低氧所致肺动脉高压，其机制与抑制肺小动脉PCNA表达、增加肺小动脉iNOS表达水平有关。
Objective To investigate the effects of angelica solution on chronic hypoxic pulmonary hypertension in rats. Methods Thirty SD rats were randomized into normoxic group,hypoxic group and angelica solution-protected group. The model of rat chronic hypoxic pulmonary hypertension was made by method of isobaric hypoxia. Angelica solution were injected before hypoxia, while the other two groups were injected normal saline. After 28 d of hypoxia, pulmonary artery pressure were measured. Expressions of prolifera- ting cell nuclear antigen （PCNA） and inducible nitric oxide synthase （iNOS） in pulmonary artery were detected by immunohistochemical staining. The index of wall thickness of rat pulmonary arteriole-percentage of the wall area in the total vascular area（ wA% ） were measured by a computerized image analyzer. Results The mean pulmonary artery pressure （mPAP） of normoxic group, hypoxic group and angelica solution-protected group werel0. 50 ± 1.90,35.36 ± 9. 11,18. 32 ± 2. 30 （ mm Hg） ; wA% of the three groups were 52. 71 ± 5.16,82. 38 ~ 8.43,64. 58 ± 9. 54 （ % ）, mPAP and wA% were significantly higher in the hypoxic group than those in the normoxic group （ P 〈 0. 01 ） and angelica solution-protected group （ P 〈 0. 01 ）. PCNA expression of normoxic group, hypoxic group and angelica solution-protected group were 3.15 ± 1.10, 24. 50 ±5.72,12. 67 ± 3.46 （ % ）. The PCNA expression in the pulmonary artery was significantly higher in the hypoxic group than those in the normoxic group （P 〈 0.01 ） and in the angelica solution-protected group （P 〈0. 01 ）. iNOS expression of normoxic group, hypoxic group and angelica solution-protected group were 2. 13 ± 1.01,17.33 ± 3.53,37.50±7.04 （%）. iNOS expression in the pulmonary artery was higher in the hypoxic group than those in normoxic group （ P 〈 0. 01 ）, and angelica significantly increased iNOS expression in comparison with the normoxic and hypoxic groups （ P 〈 0. 01 ）. Conclusion Angelica solution alleviates sion in comparison with the normoxic and hypoxic groups （ P 〈 0. 01 ）. Conclusion Angelica solution alleviates chronically hypoxia induced pulmonary hypertension in rats by inhibiting the espression of PCNA in pulmonary artery and up-regulating the expression of iNOS.
出处
《中国小儿急救医学》
CAS
2011年第3期249-251,共3页
Chinese Pediatric Emergency Medicine
基金
2010年深圳市科技计划资助项目（201002160）
关键词
当归
肺动脉高压
增殖细胞核抗原
诱导性一氧化氮合酶
大鼠
Angelica
Pulmonary hypertension
Proliferation cell nuclear antigen
Inducible nitric oxide synthase
Rat
