摘要
目的:探讨厄洛替尼联合survivin siRNA对人肺腺癌细胞A549的作用。方法:survivin siRNA转染、厄洛替尼单独或联合应用于人肺腺癌细胞A549,应用四甲基偶氮唑蓝(MTT)比色法观察对A549细胞的增殖抑制作用,免疫组化染色法观察A549细胞Survivin蛋白的表达情况。结果:survivin siRNA转染联合厄洛替尼对A549细胞具有明显的抑制作用,且可明显降低A549细胞Survivin蛋白的表达,与单独应用survivin siRNA转染和厄洛替尼比较均有统计学意义(P<0.01)。结论:应用siRNA沉默survivin表达可提高人肺腺癌细胞A549对厄洛替尼的敏感性。
Objective：To investigate the effects of erlotinib combined survivin siRNA on proliferation of human lung adenocarcinoma cell line A549.Methods：The lung adenocarcinoma cell line A549 was treated with survivin siRNA transfection and erlotinib alone or in combination.MTT method was used to observe the inhibitory effects of proliferation on A549 cells,and immunohistochemical staining to observe expression of Survivin protein in A549 cells.Results：Erlotinib combined survivin siRNA had obvious inhibitory effects on A549 cells,and could obviously decreased expression of Survivin protein in A549 cells.There had obvious differences between erlotinib combined survivin siRNA and erlotinib or survivin siRNA alone（P0.01）.Conclusions：Survivin siRNA silence the expression of survivin can enhance sensitivity of human lung adenocarcinoma cancer cell line A549 to erlotinib.
出处
《承德医学院学报》
2011年第2期122-124,共3页
Journal of Chengde Medical University
