摘要
目的对流动相的配方进行改良，建立反相HPLC法测定人血浆中阿昔洛韦浓度。方法采用SphericorbC18色谱柱，以2．5％的乙腈水溶液（内含10mmol/L高氯酸，5mmol/L乙酸钠，3．5mmol/L庚烷磺酸钠）为流动相，紫外检测波长254nm。血浆以高氯酸沉淀后高速离心取上清液分析。结果阿昔洛韦峰和其它杂峰得到很好的分离，最低检测限为30ng/ml（进样50μl，信噪比为3：1）。结论本法快速、简便、灵敏度高，且可检测到阿普洛韦代谢物。
Objective To modify the composition of mobile phase and to estabish themethod of high - performance liquid chromategraphy (HPLC) for determination of Acyclovir inhuman plasma. Methods Acyclolir was extracted from plasma by perchloric acid precipitationof protein, the supermatant was analysed on a spherisorb C18 column using wate solution of ace-tonitrile(containing 5mmol/L sodium acetate bufffer with 10mmol/L perchloric acid and 3.5mmol/L sodium heptanesulphonate ), pH6. 4 (2. 5/97, 5, V/V ) as mobile phase, followed bydetection at 254nm. Results The detection limit in plasma was 30 ng/ml 50μl injection, signal-noise ratio= 3: 1). The peak of Acyclovir was well separated from other peaks. ConclusionsThis method is rapid, simple, easy and highly sensitive. The metabolites of Acyclovir can bedetected.
出处
《江西医学检验》
1999年第3期140-142,共3页
Jiangxi Journal of Medical Laboratory Sciences
