摘要
[Objective] The research aimed to study the optimum liquid fermentation condition of Trichoderma hamatum Th12 strain,which provided the theory basis for studying and developing the biological control agent of Trichoderma spp.[Method] By using the diluted soil plate method and the plate confrontation method,Trichoderma hamatum Th12 strain was identified.Moreover,the influences of carbon source,nitrogen source,fermentation time,fermentation temperature,initial pH,inoculation amount,bottling capacity and shaker speed on the mycelium yield of Trichoderma hamatum Th12 were studied.[Result] The optimum liquid fermentation conditions of Trichoderma hamatum Th12 strain were：glucose as the carbon source,peptone as the nitrogen source,fermentation temperature was 25 ℃,initial pH was 6,inoculation amount was 0.8 ml,bottling capacity was 50 ml,shaker speed was 180 r/min.Trichoderma hamatum Th12 strain was cultivated 3 d under the condition,and the mycelium yield was the highest.[Conclusion] Under the optimum liquid cultivation condition,Trichoderma hamatum Th12 strain could provide the mycelium amount which was used for preventing and controlling Fusarium oxysporum and Rhizoctonia solani of lawn to the utmost extent.
[目的]研究钩状木霉Th12菌株最适的液体发酵条件,为研发和开发生物防治木霉制剂提供理论基础。[方法]采用稀释土壤平板法以及平板对峙法筛选、鉴定出钩状木霉Th12菌株,并考察了碳源、氮源、发酵时间、发酵温度、初始pH、接菌量、装瓶量、摇床转速对木霉菌丝产量的影响。[结果]木霉Th12菌株的最适液体发酵条件为以葡萄糖为碳源、以蛋白胨为氮源、发酵温度为25℃、初始pH为6、接菌量为0.8ml、装瓶量为50ml、摇床转速为180r/min的培养液中培养3d,菌丝产量最高。[结论]木霉Th12菌株在最佳液体培养条件中能最大限度地提供用于草坪镰孢枯萎病和褐斑病病害防治的菌丝体量。
