摘要
目的 为防止临床耐万古霉素耐甲氧西林金黄色葡萄球菌(M RSA)的产生。方法 采用4 倍万古霉素(VNC) MIC和去甲万古霉素(NVNC) MIC 分别对M RSA 进行体外耐药诱导;以琼脂双倍稀释法测定诱导前、后细菌药敏。结果 20 株MRSA 分别经4 倍VNC M IC 和NVNC MIC 非连续性诱导培养20 代,无耐VNC 或NVNC M RSA 变异株产生;但经10 次以上诱导培养后,VNC和NVNC分别对MRSA 的抗生素后效作用消失。结论 VNC 或NVNC诱导产生耐VNC MRA 或耐NVNC MRSA 相当困难,但随着培养诱导次数增多,MRSA对VNC、NVNC或NVNC能产生一定耐受性改变。提示随着VNC临床实用价值增高、范围扩大及次数增多,诱导产生耐VNC或NVNC MRSA 可能性会越来越大。
OBJECTIVE In order to control the resistance in Methicillin resistant Staphylococcus aureus (MRSA) to vancomycin (VNC) and norvancomycin (NVNC) were studied. METHODS Twenty strains of MRSA were separatedly subcultured in broth media containing four times inhibitory concentration of either VNC or NVNC. The susceptibility to antibacterial agents was tested by agar dilution method. RESULTS None of the NVC or NVNC resistant variants was seen, but the post antibiotic effects (PAES) induced by VNC or NVNC disappeared after 10 passages of culture. CONCLUSION The in vitro induction of resistance to VNC of NVNC is very difficult, but MRSA can acquire some resistance to VNC or NVNC with the increase of inducing passages. The findings suggest that it may be more likely to induce resistance in MRSA to VNC or NVNC with the increase of clinical use value, frequency and width. Therefore, it is particularly necssary to limit the clinical use of VNC or NVNC as possible.
出处
《中华医院感染学杂志》
CAS
CSCD
1999年第4期196-198,共3页
Chinese Journal of Nosocomiology
