摘要
为研究胰腺癌的转移机理，在我校原建立的人胰腺癌ＪＦ３０５ 细胞系基础上，利用细胞克隆，细胞电泳，流式细胞仪及人癌裸小鼠原位移植技术，建立了具有不同转移潜能的人胰腺癌ＪＦ３０５ 单克隆细胞亚系和具有不同转移潜能的人胰腺癌单克隆细胞亚系裸小鼠原位移植模型。结果表明该单克隆细胞亚系及其肿瘤移植模型的建立，为探讨胰腺癌转移机理提供了较理想的研究方法。
On the basis of established JF305 cell line from human pancreatic cancer at this university, cell clone technique, cell electrophoresis, flower cytometer, and cancer orthotopically implanted nude mice technique were used to establish the sublines with different metastatic potential from human pancreatic cancer line JF305 and the nude mice model implanted orthotopically with human pancreatic cancer monoclonal sublines with different metastatic potential. The results showed that the monoclonal cell sublines with different metastatic potential from human pancreatic caner JF305 and the nude mice model implanted orthotopically with the sublines, would provided a useful method to study the metastatic mechanism of human pancreatic cancer.
出处
《中国普外基础与临床杂志》
CAS
1999年第6期334-336,共3页
Chinese Journal of Bases and Clinics In General Surgery
关键词
胰腺癌
转移潜能
单克隆细胞亚系
建系
Pancreatic neoplasma Cell line Metastatic potential Cancer orthotopically implanted nude mice
