摘要
采用超声破碎和热水浸提相结合的方法提取香菇菌丝体多糖,通过L9(34)正交试验考察料液比、浸提温度、超声强度和浸提时间对多糖提取的影响,确定香菇菌丝体多糖最佳提取条件。利用D EA E-5 2离子交换和SephadexG100凝胶过滤层析纯化香菇菌丝体多糖,紫外扫描和薄膜电泳方法鉴定多糖的纯度;采用DPPH自由基、超氧阴离子自由基和羟自由基清除实验及血浆丙二醛的生成抑制作用和抑制H2O2诱导红细胞氧化溶血检测多糖的抗氧化性。结果表明:香菇菌丝体多糖最佳提取条件为料液比1:15、浸提温度90℃、超声波破碎功率400W、浸提时间3h,最适条件下鲜菌丝体的多糖提取量为3.38‰;纯化后的多糖(LMPⅡ)具有清除超氧阴离子自由基和羟自由基活性的能力,能够抑制血浆丙二醛的生成,抑制H2O2诱导红细胞氧化溶血,且呈现一定效量关系。显示纯化的香菇菌丝体多糖具有较强的抗氧化作用。
In order to determine the optimum conditions for extraction of polysaccharides from fermented mycelia of Lentinus edodes by ultrasonic cell disruption and subsequent hot water extraction,a L9（34） orthogonal array design was employed to investigate the effects of the operating parameters material-to-solvent ratio,temperature,ultrasound intensity and extraction time on polysaccharide extraction.The optimum conditions for extracting polysaccharides from fermented mycelia of Lentinus edodes were material-to-solvent ratio1：15,extraction temperature 90 ℃,ultrasound intensity 400 W and extraction time 3 h.Under the optimum conditions,the extraction yield of polysaccharides was 3.38‰.Further,the obtained extract was purified/fractionated sequentially by DEAE-52 ion exchange column and Sephadex G-100 gel permeation column and as a result,a polysaccharide having DPPH free radical scavenging ability was obtained and named as LMP Ⅱ.UV spectral scanning and cellulose acetate membrane electrophoresis demonstrated the high purity of LMP Ⅱ.The fraction had the capability of scavenging superoxide anion and hydroxyl free radicals,and could inhibit the production of MAD in plasma and hydrogen peroxide-induced erythrocyte hemolysis in a dose-dependent fashion.Thus,LMP Ⅱ has a strong antioxidant potential.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第12期19-23,共5页
Food Science
基金
辽宁省科技厅农业攻关计划项目(2009209001)
辽宁省教育厅高等学校科研项目(L2010154
2009A811)
辽宁大学"211工程"三期建设项目"现代生物技术与东北种质资源开发利用"
关键词
香菇菌丝体
多糖
纯化
抗氧化
Lentinus edodes mycelium
polysaccharides
purification
antioxidation
