摘要
为对比CTAB改良法、E.Z.N.A.TM总RNA抽提试剂盒Ⅱ、EZ-10柱式总RNA抽提试剂盒和异硫氰酸胍法4种方法抽提桑椹总RNA的效果,对该4种方法抽提后的总RNA进行纯度、得率和完整性检测以及进一步的RT-PCR检测。结果显示:CTAB改良法和E.Z.N.A.TM总RNA抽提试剂盒Ⅱ均适用于桑椹总RNA抽提,获得的总RNA纯度高、得率高、完整性好,完全可用于后续的分子生物学实验;但两者相比,CTAB改良法成本更低,操作更简单。
Four methods,including modified CTAB,E.Z.N.A.TM Total RNA KitⅡ,EZ-10 Span Column Total RNA Isolation Kit and guanidine thiocyanate,were evaluated for their performance for isolating total RNA from mulberry fruit by comparing total RNA yield,purity and completeness as well as RT-PCR results.The results showed that modified CTAB method and E.Z.N.A.TM Total RNA KitⅡ were adequate for extracting total RNA from mulberry fruit.The acquired total RNA had a good quality and could be absolutely suitable for being used for further molecular biology experiments.Besides,the modified CTAB method was more economical and easier to operate than E.Z.N.A.TM Total RNA KitⅡ.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第12期209-212,共4页
Food Science
基金
教育部大豆重点实验室开放课题(SB08A03)
关键词
桑椹
总RNA
抽提
方法比较
多酚
多糖
mulberry fruit
total RNA
extraction
comparison
polyphenol
polysaccharide
