摘要
目的了解缺氧缺血性脑损伤(HIBD)对松果体芳香烷基胺-N-乙酰基转移酶(AANAT)mRNA表达和血浆褪黑素(MLT)含量的影响,探讨松果体功能改变在HIBD中的意义。方法选取7日龄SD大鼠60只随机分成HIBD模型组和假手术组。HIBD模型组按改良Levine法建立,然后用半定量逆转录聚合酶链反应(RT-PCR)和放免技术分别测定并比较两组缺氧缺血后0 h、6 h、12 h、24 h、48 h松果体中AANAT mRNA的表达水平及血浆MLT的浓度。结果 (1)松果体中AANATmRNA的表达在HIBD后6 h、12 h、24 h低于对应假手术组(P<0.05或<0.01);假手术组各时点之间AANAT mRNA的表达水平无明显变化(P>0.05)。(2)血浆MLT浓度在HIBD后12 h、24 h均低于对应假手术组(P<0.01);假手术组各时点之间MLT浓度均无明显变化(P>0.05)。结论 HIBD早期松果体合成褪黑素的功能下降及内源性MLT保护作用的降低可能参与早期HIBD的发病。
Objective To elucidate the influences of hypoxic-ischemic brain damage（HIBD） on pineal function of melatonin synthesis and explore the possible significance of pineal function alterations in HIBD.Methods Sixty seven-day-old rats were randomly divided into 2 groups： the HIBD group and sham-operated group.Semi-quantitative reverse transcriptase polymerase chain reaction（RT-PCR） was used to measure the melatonin-synthetase—arylalkylamine-N-acetyltransferase（AANAT） mRNA expressions in pineal gland.Radioimmunoassay（RIA） was used to measure the plasma melatonin levels.Results（1） The AANAT mRNA expression 6 h,12 h,24 h after HIBD were lower than those in the corresponding sham-operated groups（P0.05 or 0.01）,and no significant difference was found among different time points in the sham-operated groups;（2） The plasma melatonin levels 12 h,24 h after HIBD were lower than those in the corresponding sham-operated groups.The melatonin levels at different time points in the sham-operated group were similar.Conclusions Pineal melatonin-synthesis is impaired on early stage of HIBD.Alternation of the protective process of endogenous MLT might be involved in pathogenic mechanism of early HIBD.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2011年第2期213-216,220,共5页
Suzhou University Journal of Medical Science
基金
江苏省卫生厅医学科技发展基金(Z200118)
苏州市社会发展指导性科技计划项目(SSZ0230)联合资助
