摘要
为提高L 天冬酰胺酶Ⅱ基因工程菌EscherichiacolipKA/CPU2 10 0 0 9的产酶量 ,采用正交试验法确定了较佳培养条件 ,并优化了发酵工艺。工程菌摇瓶产酶稳定在 190U/ml。 2 5L发酵罐产酶亦高达 180U/ml,发酵周期大大缩短 ,仅 9～ 10h。产酶量比原工艺提高 1倍以上 ,具有较好的工业生产前景。
In order to improve the enzyme production of engineering bacteria Escherichia coli pKA/CPU 210009, a suitable media has been found by orthogonal design and some fermentation conditions were optimized Enzyme production of engineering bacteria maintained 190U/ml in flasks and 180U/ml in a fermentor Fermentation period shortened greatly in a fermentor Enzyme production from the suitable media increased 2 fold than in original fermentation conditions
出处
《药物生物技术》
CAS
CSCD
1999年第3期140-143,共4页
Pharmaceutical Biotechnology
基金
国家医药管理局九五攻关项目! (960 4 3 2 0 84 )
关键词
L天冬酰胺酶Ⅱ
基因工程菌
发酵
L-asparaginase II, Engineering Bacteria, Fermentation
