摘要
目的:探讨重组Runx3腺病毒(Ad-Runx3)与顺铂联合应用对小鼠H22肝癌移植瘤的抑制作用及其机制。方法:将接种H22细胞的荷瘤小鼠随机分为对照组、Ad-Runx3组、顺铂组、顺铂+Ad-Runx3联合治疗组;15d后处死小鼠,通过称量各小鼠H22移植瘤的质量,计算抑瘤率;电镜观察荷瘤小鼠肿瘤组织超微结构改变。结果:联合治疗组对移植瘤抑制力最强,抑瘤力达65.7%,电镜下可见较为典型的细胞凋亡形态学改变。结论:Ad-Runx3与顺铂联合应用,能显著抑制小鼠肝癌移植瘤生长,这种作用可能与诱导肝癌细胞凋亡相关。
Objective:To investigate the inhibitory effect of cisplatin combined with adenovirus-mediated Runx3(Ad-Runx3) on the growth of transplanted H22 hepatocarcinoma in mice and its regulatory mechanism.Methods:The mice inoculated with H22 cells were divided into 4 groups at random: control group,Ad-Runx3 group,cisplatin group and combination group(cisplatin plus Ad-Runx3).Fifteen days later,the mice were killed,then the weight of H22xenograft tumor was measured and the tumor-inhibiting rate was calculated.Transmission electron microscopy(TEM) was used to observe the ultramicro-structure alterations of H22xenograft tumor tissue in BALB/c mice.Results:The inhibitory activity was the strongest in combination group,with the inhibition rate reaching 65.7%.TEM demonstrated the existence of apoptotic cells and apoptotic bodies in H22xenograft tumor tissue in combination group.Conclusion:Combination of cisplatin and Ad-Runx3 exerts strong inhibitory activity on the growth of transplanted H22hepatocarcinoma in mice.The effect may be correlated to the inducement of cellular apoptosis of transplanted hepatocarcinoma in mice.
出处
《长江大学学报（自然科学版）》
CAS
2011年第4期137-138,140,共3页
Journal of Yangtze University(Natural Science Edition)
基金
国家大学生创新性实验计划项目(091048930)
