摘要
从拮抗作用、定殖特性、营养竞争方面研究溶杆菌属(Lysobacter)生防菌06-4对魔芋软腐病的生防机理。结果表明:生防菌06-4对不同致病力魔芋软腐病菌可产生拮抗作用;能在魔芋根部、根际土等生态位点定殖,定殖的量维持在102～104cfu/g,定殖时间长达32d,表现出良好的环境适应性和稳定性;与魔芋软腐病原菌具有46.88%的营养物质利用相似性,具有一定的营养竞争关系。2007年田间防效测试显示,在对照病情指数为41.20的情况下,生防菌06-4对魔芋软腐病的控制效果达到58.92%。
Lysobaeter antibioticus strain 06-4 is a potential biocontrol agent against soft rot disease of Amorphophallus konjac root caused by Pectobacterium carotovora subsp, carotovora （Pcc）. Biocontrol mechanism of Lysobacter antibioticus strain 06-4 against Pcc was observed based on antagonism, colonization and nutrition competition. The results indicated that strain 06-4 showed antibacterial activity against Pcc strains with different virulence. It could colonize on the root and the rhizosphere of A.konjac and the organism could maintain 32 d with 10^2-10^4cfu/g. Nutritional requirement experiments showed that the similarity of sole-carbon-source utilization between Pcc and strain 06-4 was 46.88 %, suggesting a nutritional competition between the two microorganisms. Strain 06-4 also significantly reduced the bacterial soft rot index ofA. konjac by 58.92 % in field plot in 2007.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第3期286-289,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
农业部农业行业科研专项(3-23)
国家自然科学基金项目(30760139)
关键词
溶杆菌属
魔芋软腐病
定殖
营养竞争
Lysobacter
soft rot ofAmorphophallus konjac
colonization
nutrition competition
