摘要
[目的]探究一种高效的提取微量地衣体及地衣共生菌DNA的方法。[方法]在CTAB法的基础上,对溶液成分、比例及应用硅基质吸附柱对微量DNA提取进行优化。[结果]对于叶状、枝状和壳状地衣,采用该方法可得到质量较高的DNA。[结论]所用方法既节约样品,简便易行,费用低,稳定性好,效率高,而且不局限于新鲜材料。
[Objective] A simple,efficient method for extraction of DNA for lichens and their mycobionts was studied.[Method] Based on CTAB method,the composition,the proportion of the solution and the application of the absorption column with silica substrate were optimized.[Result] The method was successfully applied for the species from different genus,including the foliose,fruticose and crustose efficiently.[Conclusion] The method was simple,convenient,inexpensive and not restricted to fresh material.
出处
《安徽农业科学》
CAS
北大核心
2011年第15期8868-8869,共2页
Journal of Anhui Agricultural Sciences
