摘要
目的:建立小鼠佐剂性关节炎(AA)模型,研究资木瓜多糖(CSPS)对小鼠AA病理改变及炎症因子表达的影响。方法:雄性昆明鼠随机分为正常对照组、模型组、CSPS高、低剂量(分别为400,200 mg·kg-1)组、阳性药醋酸泼尼松(PAT,5.6mg·kg-1)组,每组10只。各组均于造模前1 d开始给药,2次/d,连续3 d;再于造模后第8天继续给药,2次/d,连续16 d。正常对照组、模型组ig等体积生理盐水。小鼠足部注射弗氏完全佐剂诱导建立AA动物模型,检测小鼠足爪的肿胀度,测定脾、胸腺、肝脏的指数,噻唑蓝(MTT)法检测脾淋巴细胞增殖反应,酶联免疫(ELISA)法检测脾细胞培养上清液中肿瘤坏死因子-α(TNF-α),白介素-1β(IL-1β),白介素-4(IL-4)的水平。结果:CSPS能有效减轻AA小鼠的原发性和继发性足肿胀,抑制免疫脏器指数的升高,抑制AA小鼠的脾淋巴细胞增殖反应,降低AA小鼠脾细胞TNF-α,IL-1β的产生,提高IL-4含量。结论:CSPS对AA具有抑制作用,其机制可能与抑制淋巴细胞增殖,减少促炎因子TNF-α,IL-1β释放,促进抗炎因子IL-4表达有关。
Objective：To investigate the influence of Chaenomeles speciosa polysaccharide（CSPS） on the histological changes and the production of inflammatory cytokines in mice with adjuvant arthritis（AA）.Method：Male mice were randomly divided into normal control group,model group,CSPS high and low dose groups,positive control group（n=10 in each group）.Each group was started to give medicine twice daily before model establishing for continue 3 days.After model establishing on the 8th day,medicines were given twice daily for continues 16 days.The CSPS groups were ig given the medicine 400,200 mg·kg-1 respectively.The positive control group was ig given PAT,5.6 mg·kg-1.The normal and model groups were ig given normal saline.AA was induced by intradermal injection of complete Freund′s adjuvant（CFA） into the right hind footpad of mice.Paw swelling of AA mice was measured with volume meter.The indexes of spleen,thymus and liver were analyzed.In vitro proliferation of spleen cells was examined using MTT assay.The tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β） and interleukin-4（IL-4） levels production of spleen lymphocytes were measured by ELISA.Result：CSPS significantly inhibited the primary and secondary paw swelling,and decreased immune organs indexes.The spleen lymphocyte proliferation reaction of AA mice was decreased by treatment with CSPS.CSPS markedly reduced TNF-α and IL-1β levels,and increased IL-4 level in spleen lymphocyte of AA mice.Conclusion：CSPS could suppress the arthritis of AA mice.Its active mechanism may be related to inhibition of proliferation of lymphocyte and regulation for inflammatory cytokines.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第12期159-162,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
湖北省宜昌市科技局项目(2010A01301-04)
关键词
资木瓜多糖
佐剂性关节炎
炎症
Chaenomeles speciosa polysaccharide
adjuvant arthritis
inflammation
