摘要
将样品分别置于-20℃保存和室外(20～29℃)进行光照氧化,然后利用高效液相色谱(HPLC)跟踪检测,通过气相色谱-质谱联用(GC-MS)技术对光照前后挥发油化学成分及含量的变化进行分析比较。从光照氧化前白术挥发油中鉴定出32个组分,占挥发油总成分的98.01%;从光照氧化后白术挥发油中鉴定出27个组分,占挥发油总成分的90.70%。光照氧化后增加了2个组分,消失了7个组分;含量降低的有8个,增加的有17个。含量降低最明显的组分是苍术酮和γ-榄香烯;含量增加最明显的组分是白术内酯Ⅰ、白术内酯Ⅲ、β-桉叶醇、广木香内酯、Isovelleral和17-(1,5-二甲基己基)-10,13-二甲基-2,3,4,7,8,9,10,11,12,13,14,15,16,17-十四氢化-1H-环戊[a]3-菲酚。
To investigate the effect of sunlight oxidation on the composition and content changes of volatile oil,two volatile oil samples extracted from Atractylodes macrocephala Koidz were treated by preservation at-20℃ without sunlight and sunlight oxidation at 20~29℃,respectively.High performance liquid chromatagraphy and gas chromatography-mass spectrometry were employed to analyze ingredients and their contents of the treated samples.32 and 27 components were identified in the volatile oil without sunlight oxidation and with sunlight oxidation,which account for 98.1% and 90.70% of the total components in the oil,respectively.After sunlight oxidation,2 new components were found and 7 components disappeared.8 components decreased and 7 components increased in their contents,in which Atractylon and γ-Elemene decreased remarkably,while Attractyenolide Ⅰ,Attractyenolide Ⅲ,β-Eudesmol,Costunolide,Isovelleral,and 17（1,5 Dimethylhexyl）-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1 H-cyclopen ta[a]phe-nanthren-3-ol increased dramatically.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第3期316-320,共5页
Journal of Analytical Science
基金
山西省研究生优秀创新重点项目(No.20103016)
山西省高校科技开发项目(No.2010101)
关键词
白术挥发油
光照氧化
成分分析
Volatile oil of Atractylodes macrocephala Koidz
Sunlight oxidation
Composition analysis
