摘要
目的:观察骨痹舒含药血清对IL-1β作用下体外培养的兔关节软骨细胞表达Coll-Ⅱ、MMP-1、MMP-13的影响,探讨骨痹舒防治OA的作用机制。方法:获取1月龄兔关节软骨细胞,取第Ⅰ代细胞用于实验。实验分为空白组、模型组、骨痹舒组、抗骨增生组4组,培养第4d时,模型组、骨痹舒组及抗骨增生组分别加入IL-1β10ng/mL继续培养,分别在培养后24、48、72h用In-cell Western法检测各蛋白表达情况。结果:各时间点空白组与模型组Coll-Ⅱ、MMP-1及MMP-13表达差异均有统计学意义(P<0.05),骨痹舒组较模型组Coll-Ⅱ表达在48、72h时间点有显著性差异(P<0.05),MMP-1及MMP-13表达在各时间点均有显著性差异(P<0.05)。结论:骨痹舒能有效抑制IL-1β对Coll-Ⅱ的降解和破坏作用,能抑制IL-1β对MMP-1、MMP-13的诱导和激活作用。
Objective： To investigate the effects of Gubishu medicated serum on the expression of Coll-Ⅱ,MMP-1,MMP-13 of rabbit articular chondrocytes stimulated by interleukin-1beta.Methods： Articular chondrocytes were obtained from the cartilage of 1-month rabbit and cultured in vitro.The passageⅠchondrocytes were used.They were divided into 4 groups： control groups,model groups,Gubishu groups and Kanggu Zengsheng groups.After 4 days cultured,IL-1β 10ng/mL were added in the control groups,Gubishu groups and Kanggu Zengsheng groups.In cell western blot assay method was adopted to observe the influence of every different groups on the expression of Coll-Ⅱ,MMP-1 and MMP-13 of chondrocytes stimulated by interleukin-1beta after incubated 24h,48h and 72h.Results： The expression of Coll-Ⅱ,MMP-1and MMP-13 of chondrocytes were significant differences between control groups and model groups at same time point（P0.05）;The expression of Coll-Ⅱof chondrocytes were significant differences between Gubishu groups and model groups at 48h,72h（P0.05）;There was significant differences in expression of MMP-1and MMP-13 at same time point（P0.05）.Conclusion： Gubishu medicated serum can significantly inhibit the degradation and destroy of Coll-Ⅱcaused by IL-1β,also inhibit the IL-1β-induced MMP-1,MMP-13.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第6期1426-1428,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
