摘要
目的通过观察艾灸对佐剂性关节炎大鼠免疫介导物质β-内啡肽含量的影响,揭示针灸调节免疫功能的机理。方法将40只大鼠分为空白组、模型组、灸命门组、灸关元组,灸非穴组,除空白组外,其余大鼠于右后足跖皮内注射弗氏完全佐剂造模,模型组造模后不做任何处理,灸命门组灸命门穴、灸关元组灸关元穴、灸非穴组灸关元穴左侧旁开0.5cm处。观察足爪肿胀率并观测血浆、下丘脑、垂体和淋巴结中β-内啡肽(β-EP)含量。结果与模型组比较,灸命门组与灸非穴组右足足爪肿胀率明显降低(P<0.01);与灸命门组比较,灸关元组与灸非穴组右足足爪肿胀率明显升高(P<0.05)。与空白组比较,模型组左足足爪肿胀率明显升高(P<0.05),灸命门组与灸关元组左足组爪肿胀率明显升高(P<0.05);与模型组比较,灸命门组、灸关元组、灸非穴组左足足爪肿胀率明显降低(P<0.01)。与空白组比较,模型组、灸命门组、灸关元组、灸非穴组大鼠血浆中β-EP含量明显降低(P<0.05);与空白组比较,模型组大鼠下丘脑中β-EP含量明显升高(P<0.05);与空白组比较,灸关元组、灸非穴组大鼠垂体中β-EP含量明显升高(P<0.05);与模型组比较,灸关元组、灸非穴组大鼠垂体中β-EP含量明显升高(P<0.05);与模型组比较,灸命门组、灸关元组、灸非穴组大鼠淋巴结中的β-EP含量明显升高(P<0.05)。结论灸命门穴和非穴对右足原发性炎症有抑制作用;灸命门穴、关元穴、非穴对左足继发性炎症有抑制作用。推测艾灸上述穴位对机体免疫炎症的调整作用是通过提高淋巴结β-EP含量、调动中枢内β-EP而实现的。
Objective To study the mechanism of moxibustion in immunoregulation through observing on the influence of moxibustion on content of β-endorphin （β-EP） in rats with adjuvant arthritis.Methods All 40 rats were divided into the blank group,model group,Mingmen （GV4） group,Guanyuan （CV4） group and non-acupoint group.Except the blank group,the rats in other groups were injected Freund＇s complete adjuvant in the right posterior leg for modeling.The Mingmen group was given moxibustion on GV4,Guanyuan group,on CV4,and non-acupoint group,on 0.5 cm lateral to CV4 in the left.The rate of paw edema and the content of β-EP in plasma,hypothalamus,pituitary and lymph nodes were observed.Results The rate of right paw edema decreased significantly in the Mingmen group and non-acupoint group compared with the model group （P〈0.01）.The rate of right paw edema increased significantly in the Guanyuan group and non-acupoint group compared with the Mingmen group （P〈0.05）.The rate of left paw edema increased significantly in the model group（P〈0.05）,Mingmen group and Guanyuan group（P〈0.05） compared with the blank group.The rate of left paw edema decreased significantly in the Mingmen group,Guanyuan group and non-acupoint group compared with the model group （P〈0.01）.The plasma content of β-EP decreased significantly in the model group,Mingmen group,Guanyuan group and non-acupoint group compared with the blank group （P〈0.05）.The hypothalamus content of β-EP increased significantly in the model group compared with the blank group （P〈0.05）.The pituitary content of β-EP increased significantly in the Guanyuan group and non-acupoint group compared with the blank group （P〈0.05）.The pituitary content of β-EP increased significantly in the Guanyuan group and non-acupoint group compared with the model group （P〈0.05）.The content of β-EP in lymph nodes increased significantly in the Mingmen group,Guanyuan group and non-acupoint group compared with the model group （P〈0.05）.Conclusion The moxibustion on GV4 and non-acupoint can inhibit the primary inflammation of the right leg,and that on GV4,CV4 and non-acupoint can inhibit the secondary inflammation of the left leg.It is inferred that the regulation to immune inflammation by moxibustion on the above mentioned acupoints maybe related to the increase of β-EP content in lymph nodes and arousing of central β-EP content.
出处
《北京中医药大学学报（中医临床版）》
2011年第3期1-4,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京市自然科学基金资助项目(No.7052037)
