摘要
为建立液相色谱-质谱联用（LC-MS/MS）检测番茄中苯醚甲环唑残留的方法,该研究优化了样品前处理以及LC-MS/MS质谱条件（离子化方式为电喷雾电离,以母离子m/z 406.1以及裂解离子m/z 251和m/z 337进行定性分析,定量离子为m/z 251）。结果显示,正己烷提取番茄中苯醚甲环唑效果较好,无需净化可以直接上机检测。方法标准曲线线性良好,R2=0.998,最低检出限为2.0 ng/ml,回收率为80.5%~101.0%,RSD≤4.68%。该方法操作简便、分析时间短、灵敏度高、定性定量准确,检测效率高。
To identify and quantify the difenoconazole in tomato using the liquid chromatography/tandem triple quadrupole mass spectrometry（LC-MS/MS）,the sample preparation and the condition of the mass spectrum were optimized.Electric spray ionization was used for ionization,the parent ion m/z 406.1 and fragment ion-pair m/z 251 and m/z 337 were used for qualitative analysis,and m/z 251 was used for quantitative analysis.The result showed that n-hexane used as extract solvent was favorable for the extraction of difenoconazole without purification.Linear results was satisfactory with R2= 0.998.The limits of detection（LOD） was 2.0 ng/ml.Recoveries of the analyte,depending on the matrices and spiked concentration,ranged from 80.5 % to 101.0 % with relative standard deviations（RSD） less than 4.68%.The method developed was simple,rapid and sensitive with accuracy of qualitative and quantitative for the detection of difenoconazole
出处
《江苏农业学报》
CSCD
北大核心
2011年第3期668-671,共4页
Jiangsu Journal of Agricultural Sciences
基金
江苏省自主创新基金项目[CX(09)0101
CX(09)632]
关键词
液相色谱-质谱
苯醚甲环唑
番茄
liquid chromatography-tandem triple quadrupole mass spectrometric（LC-MS/MS）
difenoconazole
tomato
determination
