摘要
目的研究酶法提取首乌藤多糖的最佳工艺条件。方法利用酶解法提取首乌藤多糖,筛选出最佳适用酶,然后利用苯酚-硫酸法测定多糖含量,以多糖得率和多糖含量为综合评价指标,采用单因素试验对影响首乌藤多糖的提取工艺进行研究。结果首乌藤多糖的最佳适用酶为纤维素酶,由单因素试验确定的酶法提取首乌藤多糖的最佳工艺条件为pH 5.0,纤维素酶用量3%,酶解时间4 h,酶解温度55℃。结论纤维素酶可显著提高首乌藤多糖的提取率。
OBJECTIVE To study the optimal enzyme extraction technology of polysaccharides from Polygoni Multiflori Caulis.METHODS The yield of polysaccharide and polysaccharide content were used to evaluate the factor levels.Based on the optimized enzyme,the extraction conditions were optimized by the single factor tests.RESULTS The conditions of the optimal extraction process using cellulose enzyme method were as follows： the pH value was 5.0,the concentration of cellulose enzyme was 3%,the extraction time was 4 hours and the extraction temperature was 55 ℃.CONCLUSION The yield of polysaccharide extracted from Polygoni Multiflori Caulis can be significantly increased by using cellulose enzyme extraction process.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第6期519-523,共5页
Chinese Journal of Modern Applied Pharmacy
基金
河南省郑州市金水区科技攻关项目(20093543)
关键词
首乌藤多糖
酶法
提取工艺
Polygoni Multiflori Caulis polysaccharide
enzyme method
extraction process
