摘要
研究转化生长因子β1(TGF-β1)+869T/C基因多态性与甲状腺疾病的相关性。采用序列特异性引物聚合酶链反应(PCR-sequence specific primers,PCR-SSP)方法,检测136例结节性甲状腺肿伴甲亢患者,132例毒性甲状腺腺瘤患者,185例Graves病患者,202例桥本甲状腺炎患者,240例正常对照组的TGF-β1+869T/C基因多态性。结果表明:1.TGF-β1+869T/C的CC基因型及C等位基因频率分布在结节性甲状腺肿伴甲亢、毒性甲状腺腺瘤显著高于正常对照,组间差异有统计学意义(P<0.05);2.结节性甲状腺肿伴甲亢与毒性甲状腺腺瘤合称甲亢有结节组,其CC基因型及C等位基因频率分布显著高于单纯Graves病组,二者比较差异有统计学意义(P<0.05);3.有结节的HT组的TGF-β1+869T/C的CC基因型及C等位基因频率分布显著高于无结节的HT组,组间比较差异有统计学意义(P<0.05)。提示:TGF-β1基因+869T/C可能与结甲伴甲亢、毒性腺瘤的发病有关,而与GD、HT发病无关;"C"等位基因可能是包头地区汉族人群中结节性甲状腺肿伴甲亢、毒性甲状腺腺瘤的易感因素之一。CC基因型与C等位基因可能与甲状腺疾病中的结节存在相关性。
To analyse the relationship of the polymorphism of transforming growth factor β1（TGF-β1） ＋869T/C gene and thyroid disease,the polymorphisms of TGF-β1＋869T/C gene of 136 patients with nodular goiter accomplished with hyperthyroidism（HT）,132 patients with toxic thyroid adenoma,185 patients with Graves disease,202 patients with Hashimoto＇s thyroiditis and 240 cases of normal individuals were detected by PCR-sequence specific primers（PCR-SSP）,it was demonstrated that the distribution of TGF-β1 ＋869 T/C genotype and allele frequency in nodular goiter with hyperthyroidism and toxic thyroid adenoma showed a significant difference in comparison with the normal control group（P0.05）.Nodular goiter with HT and toxic thyroid adenoma were collectively known as the nodular HT group.There was significant difference between the distribution of genotype and C allele frequency with simple Graves disease group（P0.05）.Also,significant difference existed between the distribution of nodules HT group TGF-β1 869T/C genotype and C allele frequency with the non-nodules HT group（P0.05）.Thus,it may be concluded that TGF-β1 ＋869T/C gene could be related to the incidence of nodular goiter with hyperthyroidism and toxic thyroid adenoma,and but not to Graves disease.The CC-genotype HT.C allele may be one of the predisposing factors in Chinese Han population baotou with hyperthyroid nodular goiter,toxic thyroid adenoma.CC genotype and C allele may be associated with the existence of nodules in thyroid disease.
出处
《现代免疫学》
CAS
CSCD
北大核心
2011年第3期244-247,共4页
Current Immunology
