摘要
目的探讨PDCD5蛋白在胃癌及癌旁组织中表达的特点,并根据其与胃癌生物学行为之间的关系分析对胃癌诊断以及预后评估的意义。方法采用免疫组织化学SP法检测50例胃癌及癌旁组织中PDCD5蛋白,以人工阅片评分以及Image-Pro Plus 6.0图像分析软件半定量分析两种方法判断其表达。结果 PDCD5蛋白在胃癌组织中的阳性表达率(22.0%)明显低于癌旁组织(76.0%),并且在胃癌组织中的阳性率与分化程度(P<0.01)、浸润度(P<0.05)、淋巴转移(P<0.05)相关。图像软件半定量分析结果显示PDCD5在胃癌组织中表达IOD为(8033.83±7719.14),显著低于癌旁组织(50224.35±39934.31),并且不同类型胃癌组织中IOD水平与分化程度、浸润度、淋巴转移相关之间存在显著相关性(P<0.01)。结论人工阅片评分及图像分析软件半定量分析两种方法结果一致,PDCD5蛋白低表达于胃癌组织,在不同分化程度、浸润度以及有无淋巴转移组间的表达都有显著差异。PDCD5蛋白低表达预示肿瘤有较强的侵袭性,可用于判断胃癌预后。
Objective To explore the role of PDCD5 protein in diagnosis and prognosis of gastric cancer through the analysis of the relationships between PDCD5 protein and biological behavior of cancer.Methods Immunohistochemical staining（SP method） for PDCD5 was performed in 50 cancer tissues and 50 adjacent tissues samples,the expression level of PDCD5 protein was analysed by microscope ratings and Image-Pro Plus 6.0 software.Results The positive rate of PDCD5 protein in gastric cancer tissues（22.0%） was significantly lower than that in adjacent tissues（76.0%）.The positive expression rate in cancer tissue was related to degree of tumor differentiation（P0.01）,depth of tumor invasion（P0.05） and lymphatic metastasis（P0.05）.Semiquantitative analysis showed that the value of IOD in gastric cancer tissues（8033.83±7719.14） is significantly lower than that in adjacent tissues（50224.35±39934.31）.And there was a significant correlation between the IOD level and the degree of differentiation,infiltrating and lymph metastasis（P0.01）.Conclusion The results of microscope ratings were consistent with image analysis software.The marked difference of PDCD5 protein expression between gastric cancer and normal samples was observed.Lower expression of PDCD5 indicated stronger invasive tumors and PDCD5 protein might be a biomarker for prognosis of gastric cancer.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第5期532-534,共3页
Cancer Research on Prevention and Treatment
基金
吉林省教育厅"十一五"资助项目(2008405)
关键词
胃癌
PDCD5
诊断
预后
Gastric cancer
PDCD5
Diagnosis
Prognosis
