摘要
采用水杨酸作内标,以甲醇∶水(80∶20)为流动相,紫外302 nm 为检测波长,建立了六茜素含量测定的反相高效液相色谱法。其对六茜素的最低检测质量浓度为0.5 m g/L,六茜素质量浓度Y在1～80 m g/L范围内与六茜素峰高和内标峰高的比 X 成良好的线性关系,回归方程为Y = 29.699X + 0.045,r =0.999 9。日内与日间精密度分别为0.97% 和1.01% 。对3 批六茜素样品的含量测定结果表明,该方法简便、快速、准确。
A high performance liquid chromatograph method for the quantitative determination of pyroline (2 methylhydroquinone) was established. The samples were added salicylic acid as an internal standard and chromatographed on a reversed phase IRREGULAR H C 18 column with UV detection at 302 nm. The mobile phase was CH 3OH H 2O=80∶20. The column temperature was 20℃ and flow rate was 1.0 mL/min. The calibration curve was Y=29.699X+0.045, r=0.999 9. It was linear within the concentration range of 1.0 80.0 mg/L and minimum detectable concentration was 0.5 mg/L. Intra day and inter day RSD were 0.97% and 1.01%, respectively. By using this method, the concentration of pyroline in the samples were determined. The method has been proved to have the advantage of easy, fast and precise.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第6期594-595,共2页
Chinese Journal of Veterinary Science
基金
"九.五"国家科技攻关项目子
中国农业科学院重大推广基金
关键词
高效液相色谱法
六茜素
中草药
high performance liquid chromatograph
pyroline
quantitative determination
