摘要
青枯病是当前设施辣椒主要的土传病害之一。为了选择抗性砧木和探讨嫁接辣椒的抗病机理,通过人工接种青枯病原菌,分析了不同辣椒品种砧木的发病率和病情指数变化,结果表明:‘富根卫士’(简称‘卫士’)发病晚,发病率低,病情指数最小,抗病性最强。以‘卫士’辣椒为砧木,以‘新丰2号’为接穗进行嫁接,以接穗自根嫁接为对照,研究其发病率、病情指数和渗透调节物质的变化。结果表明:嫁接植株的发病率和病情指数显著低于对照,接种20d时,嫁接植株发病率和病情指数分别比对照低33%和43%。接种前嫁接的植株根系和叶片的含水量、水势、渗透势均低于对照,束缚水/自由水比值、可溶性糖及脯氨酸含量均高于对照。接种后,辣椒根系和叶片的含水量、水势、渗透势均逐渐降低,束缚水/自由水比值逐渐升高,可溶性糖和脯氨酸含量先升高后降低;与对照相比,嫁接植株根系和叶片的含水量、水势、渗透势的降低幅度及束缚水/自由水比值升高幅度明显较小,可溶性糖和脯氨酸含量显著增加。研究结果表明嫁接可显著提高辣椒青枯病的抗性,其抗病机理与渗透调节能力增强有关。
Bacterial wilt is one of the primary diseases of pepper grown in solar-greenhouse. In order to select an resistant rootstock and elucidate the resistant mechanism of grafted pepper,changes of disease incidence and disease index of bacterial wilt in different rootstock varieties of pepper were investigated by manual inoculation,with‘Xinfeng 2’as control. The result showed that‘Weishi’was diseased in later days,and showed the lowest disease incidence and disease index,therefore represent the highest disease resistance among the four rootstock varieties and the control. Changes of disease incidence and disease index of bacterial wilt and osmoregulation in grafted（‘Xinfeng 2’scion grafted onto‘Weishi’）and own-root plants（‘Xinfeng 2’,control）of pepper were investigated after being inoculated. The results showed that the disease incidence and disease index of grafted plants were lower than those of control. Measured on 20 d after inoculation,the disease incidence and disease index of grafted plants were lower by 33% and 43% respectively compared with the control. Before inoculation,grafted plants showed lower water content,water potential and osmotic potential,but higher bound water/free water,soluble sugar and proline content in roots and leaves,compared with the control. The water content,water potential and osmotic potential in roots and leaves of pepper plants decreased gradually after inoculation,but bound water/free water increased,the soluble sugar and proline contents increased at early stage,but decreased later. Compared with control plants,the grafted peppers showed less decreasing rates in water content,water potential and osmotic potential,and less increasing rate in bound water/free water. Soluble sugar and proline contents in leaves and roots of grafted peppers were significantly higher than those of control plants. These data indicated that graft significantly increased the bacterial wilt resistance in pepper,and is closely related to the osmoregulation.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第5期903-910,共8页
Acta Horticulturae Sinica
基金
山东省科技发展计划项目(2010GNC10901)
‘十二五’国家科技支撑计划项目(2011BAD12B03)
关键词
辣椒
砧木
病情指数
束缚水
自由水
渗透调节
pepper
rootstock
disease index
bound water
free water
osmoregulation
