摘要
目的:探讨硫化氢(H2S)对急性心肌缺血大鼠线粒体功能的影响,并探讨其改善急性心肌缺血损伤的作用机制。方法:通过结扎大鼠左冠状动脉前降支建立急性心肌缺血模型。雄性SD大鼠48只随机分为6组(n=8):假手术组,缺血组,缺血+硫氢化钠(NaHS)低、中、高剂量组和缺血+炔丙基甘氨酸(PPG)组。透射电镜观察心肌组织线粒体超微结构;检测血浆中H2S含量、心肌组织CSE活性;测定心肌线粒体活力、膜肿胀度及线粒体总ATP酶、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)的活性和丙二醛(MDA)含量。结果:与假手术组比较,缺血组大鼠血浆H2S含量和心肌组织中CSE活性降低;心肌线粒体膜肿胀,线粒体活力下降;线粒体中MDA含量明显升高,ATP酶、SOD、GSH-Px活性明显降低(P<0.01)。与缺血组比较,缺血+NaHS低、中、高剂量组大鼠血浆H2S含量和心组织中CSE活性均升高;缺血+NaHS中、高剂量组大鼠心肌线粒体MDA含量明显减少,膜肿胀度减轻;缺血+NaHS低、中、高剂量组线粒体活力有所恢复,ATP酶、SOD、GSH-Px的活性明显升高(P<0.05或P<0.01)。PPG可部分减弱H2S的心肌保护作用(P<0.05或P<0.01)。结论:H2S可增强线粒体ATP酶、SOD、GSH-Px的活性,降低线粒体脂质过氧化水平,从而起到对大鼠急性心肌缺血的保护作用。
Objective：To investigate the effect of hydrogen sulfide（H2S） on mitochondrial function in acute myocardial ischemia in rats.Methods：Acute myocardial ischemia models were established by ligating the left anterior descending coronary artery（LADC） of rats.Fourty-eight male SD rats were randomly divided into 6 groups（n=8）：sham operation group,ischemia group,ischemia ＋ sodium hydrosulfide（NaHS） low,middle and high dose groups and ischemia ＋DL-proparglycine（PPG） group.The ultrastructures of myocardial mitochondria were observed with electron microscope.The content of H2S in plasma and the activity of cystathionine-γ-lyase（CSE） in myocardial tissue of rats were respectively detected.The swelling and activity of myocardial mitochondria were determined.The activities of ATPase,GSH-PX,SOD and the content of malondial-dehyde（MDA） in myocardial mitochondria of rats were also measured.Results：Compared with those of the sham operation group,the content of H2S in plasma,the activity of CSE in myocardial tissue and the activity of myocardium mitochondria were significantly decreased.The activities of ATPase,SOD,GSH-Px in myocardial mitochondria were significantly decreased,The content of malondial dehyde（MDA） in myocardial mitochondria and the swelling of mitochondria were distinctly increased in the ischemia group（P0.01）.Compared with those of the ischemia group,the content of H2S in plasma and the activity of CSE in myocardial tissue were increased,and the activities of mitochondria,ATPase,SOD,and GSH-Px in myocardial mitochondria were significantly increased in ischemia ＋ NaHS low,middle and high-dose groups;the swelling of mitochondria and the content of MDA in myocardial mitochondria were significantly decreased in ischemia ＋ NaHS middle and highdose groups（P0.05 or P0.01）.The administration of PPG could partially reduce the myocardial protection of hydrogen sulfide（P0.05 or P0.01）.Conclusion：It could be concluded that the administration of hydrogen sulfide could enhance the activities of mitochondrial ATPase,SOD,GSH-Px,decrease the level of mitochondrial lipid peroxidation,and play a protective effect against acute myocardial ischemia.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2011年第2期158-162,共5页
Chinese Journal of Applied Physiology
基金
河北省自然科学基金资助项目(C2009001458)
关键词
硫化氢
急性心肌缺血
线粒体
大鼠
hydrogen sulfide
acute myocardial ischemia
mitochondria
rats
